Harnessing the mechanisms of fungal bioluminescence to confer autonomous luminescence in plant and animal cells

This discovery is a toward figuring out whether can natively produce all the molecules required for light emission. It also means that the glow of bioluminescent plants can now be more closely aligned with their internal biology.

The technology reported in the paper is a hybrid that couples the newly found plant hispidin synthases to other necessary bioluminescence enzymes found in mushrooms. This hybrid pathway allows the subtle inner rhythms and dynamics within plants to be unveiled as an ever-changing display of living light.

"This technology is a plug-and-play tool to visualize virtually any molecular physiology at the organismal level, completely non-invasively," Sarkisyan states. His work also revealed that not only does a single indigenous plant gene effectively substitute for two , the plant gene is notably smaller and has simpler biological requirements for luminescence. The gene's reduced size also enhances its usability and flexibility, making it more adaptable for extended applications.

This research was sponsored by Light Bio, a plant synthetic biology company co-founded by Sarkisyan, which aims to transform the horticulture industry with beautiful biotech creations, such as glowing plants. The first product to exploit the hispidin-based pathway is Firefly Petunia, so named because its bright light-emitting flower buds resemble fireflies.

By exploiting the hispidin synthase pathway in plants, MRC Laboratory of Medical Sciences scientists have created Chrysanthemums that glow in the dark. Credit: LightBio

MRC Laboratory of Medical Sciences synthetic biologist Karen Sarkysian observes his glowing plants which could one day be used to signal health or disease. Credit: LightBio

By exploiting an enzyme pathway found in bioluminescent fungi, MRC-LMS scientist Karen Sarkysian has created glow in the dark Firefly Petunias️ with biotech company LightBio. Credit: LightBio