A single-domain antibody that specifically recognizes amyloid-beta 42 oligomers

January 6th, 2014
A single-domain antibody that specifically recognizes amyloid-beta 42 oligomers
The anti-beta-amyloid monoclonal antibody 6E10 could recognize all types of Aβ42, but clone 10A1 and 11F5 only bound to tetramers and octamers. The helper phage M13K07 was used as a negative control. Credit: Neural Regeneration Research
Earlier amyloid-beta assemblies may be one of the most important causes of Alzheimer's disease. Passive immunization of anti-amyloid-beta antibodies can reduce amyloid-beta burden and improved memory performance in various Alzheimer's disease animal models or patients. Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers.

Thus, a recent study published in the Neural Regeneration Research (Vol. 8, No. 33, 2013) successfully constructed a naïve human Fab library and obtained a human Fab antibody and a single-domain antibody that specifically recognized amyloid-beta (Aβ)42 oligomers.

More information:
Yuan ZN, Du MG, Chen YW, Dou F. Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers. Neural Regen Res. 2013;8(33):3107-3115.

Provided by Neural Regeneration Research

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