Sleeping sickness parasite masters three different swimming modes

June 21, 2011 in Biology / Cell & Microbiology
In the case of Trypanosoma the flagellum is connected to the cell on its entire length. © MPIDS

( -- The causative agent of African sleeping sickness, annually responsible for several thousands of deaths in Africa and South America, is a motile cell: it propels itself through its host’s bloodstream until – in the last stage of the disease – it overcomes the blood-brain-barrier and penetrates its victim’s brain. In order to fight this deadly disease, scientists are trying to understand the parasite’s exact patterns of movement. Researchers from the Max Planck Institute for Dynamics and Self-Organization (MPIDS) in Germany as well as from the Universities of Würzburg, Göttingen and Basel have now succeeded in identifying three different swimming modes. In addition, they were able to show for the first time, that these swimming modes correspond to the shape and stiffness of the parasite.

Cells that move independently are not uncommon: E. coli bacteria wrap their flagellum up into a tight bundle in order to propel themselves forward; sperm cells move by whipping their tails back and forth. In the case of Trypanosoma, parasites that cause , the exact motion-sequence is not yet known. The reason for this is their much more complicated “physique”: The flagellum, most probably the cell’s main motor, does not link to the cell body like a tail as is the case with sperm. Instead, it is connected to the cell on its entire length (see figure 1). What is for sure, however, is that Trypanosoma’s method of moving forward is extremely efficient: The parasites swim through their victim’s blood stream with velocities ranging from 20 to 40 μm/s.

The German-Swiss group of scientists was now able to show that Trypanosoma brucei brucei, the parasite that infects cattle, swims in three different swimming modes. Under a microscope the researchers tracked the exact paths chosen by the cells in a period of a few seconds within a nutrient solution and analyzed these statistically. As it turned out, the parasites could be divided into three groups: while some of them moved into the same direction for several seconds, others tumbled randomly every which way. “As a result, they hardly move forward at all”, Sravanti Uppaluri from the MPIDS describes this group. A third group switched between both swimming modes.

In order to study why a certain cell favours a certain “gait”, an even more precise – and especially quicker – look at Trypanosoma was necessary. “First, we had to track a cell for a certain period of time and identify its swimming mode”, explains Uppaluri who performed the challenging analysis. In a next step, the scientists took a closer look at the anatomical properties of the cells with the help of a high-speed camera, allowing for up to several thousands of images per second and a higher spatial resolution. By measuring the distance between both cell ends the researchers found that the directional swimmers have a stretched form indicating they are stiffer than their tumbling counterparts. The randomly tumbling Trypanosoma on the other hand proved to be rather more bent, pointing to a more flexible body.

A reason for these anatomical differences within a single population of Trypanosoma is yet unclear. “It is possible, that they stand for different phases within the parasite’s life cycle”, says Thomas Pfohl from the University of Basel. The directional motion could belong to the last phase, when the agent drills itself into its victim’s tissue. Another explanation could be that when looking for food, the cells choose the directional swimming strategy.

Top: The randomly tumbling Trypanosoma have a more bent shape pointing to a more flexible body. Bottom: The directional swimmers have a stretched form indicating that they are stiffer © MPIDS

A few years ago, the researchers from Würzburg together with their colleagues from MPIDS discovered that swimming quickly helps the Trypanosomae avoid host antibodies. The current that passes along the parasites’ smooth surface pulls the antibodies downstream into the direction of the cell mouth, where they are then “eaten”. In a next step, the scientists now want to study which swimming mode is especially favourable for this survival strategy. “Possibly, the tumbling Trypanosoma are more vulnerable than their streamlined colleagues”, says Pfohl. A precise understanding of these motions could therefore help find a starting point for fighting the systematically.

More information: Sravanti Uppaluri, Jan Nagler, Eric Stellamanns, Niko Heddergott, Stephan Herminghaus, Markus Engstler, Thomas Pfohl Impact of microscopic motility on the overall swimming behaviour of parasites, PloS Computational Biology, June 16th, 2011

Provided by Max-Planck-Gesellschaft

"Sleeping sickness parasite masters three different swimming modes" June 21, 2011