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New testing method detects whether bacteria respond to antibiotics in five minutes

bacteria colony
Credit: Pixabay/CC0 Public Domain

Researchers at Karolinska Institutet in Sweden have developed a molecular method able to detect whether or not bacteria respond to antibiotics within minutes. The findings are presented in the journal Nature Microbiology and the researchers now hope to develop a simple test for doctors to use.

"We are confident and hope that this can be one of many tools that doctors need to tackle antibiotic resistance, which is a serious and growing problem," says principal investigator Vicent Pelechano, associate professor at the Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet.

The method is called 5PSeq, is simple to use, and is based on sequencing the messenger RNA (mRNA) that the bacteria break down as they synthesize proteins. The measurements reveal how the bacteria are affected by different environmental factors, such as and other types of stress.

Rapid test for clinical use

The researchers tested the method on a total of 96 from different phyla in complex clinical samples taken, for example, from fecal matter and the vagina, but also in compost samples. After only a matter of minutes, they were able to see whether or not the bacteria responded to antibiotic treatment; the effect was most salient after about half an hour.

3N Bio is a company that Dr. Pelechano and some of his colleagues have started up in the KI Innovations incubator to develop the method and create a rapid molecular test for . They have now received financing from the Swedish Research Council to demonstrate proof of concept for such a test in collaboration with the Karolinska University Hospital.

"It's crucial that doctors can quickly find the right antibiotics for seriously ill patients with bacterial infections to reduce the unnecessary use of ," says Dr. Pelechano. "Current methods of testing antibiotic resistance can take hours or even days, but often treatment needs to be given more promptly than that to avoid serious consequences for the patient. Because of this, a is often prescribed, which increases the risk of resistance."

Apart from measuring , the method can be used to help researchers understand how bacteria handle stress and interact with each other and with their hosts. The researchers will continue to study complex gut samples to examine how the bacterial communities interact in our gut and contribute to health and disease.

More information: Vicent Pelechano, Atlas of mRNA translation and decay for bacteria, Nature Microbiology (2023). DOI: 10.1038/s41564-023-01393-z. www.nature.com/articles/s41564-023-01393-z

Journal information: Nature Microbiology

Citation: New testing method detects whether bacteria respond to antibiotics in five minutes (2023, May 22) retrieved 21 February 2024 from https://phys.org/news/2023-05-method-bacteria-antibiotics-minutes.html
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