What purpose do reduced mitochondria serve?

What are reduced mitochondria for?
Graphical abstract. Credit: Authors of the study

A few years ago, scientists discovered a curious case of protozoa (oxymonads) that do not have mitochondria. Since then, the research group of Associate Professor Hampl from the Faculty of Science of Charles University and the BIOCEV research center has been asking how the unique loss of mitochondria occurred.

In a paper published in the journal Current Biology, Associate Professor Hampl's team, with Justyna Zítek as the first author, used the closest known relative of the oxymonads, a free living flagellate known as Paratrimastix pyriformis, as a model species. This species does have , but they are greatly reduced. The team focused on the appearance of the closest relative of the lost mitochondria, and what this reduced mitochondria actually provides for the organism. In this way, scientists can learn how the reduction of mitochondria proceeded, what makes the oxymonads so unique that they were able to get rid of the mitochondria altogether.

One of the key functions of mitochondria is the synthesis of iron-sulfur clusters, which almost always starts there, even in organisms that have greatly simplified these organelles, such as Giardia intestinalis. A few years ago, Hampl's team showed that a whole broader group of oxymonads, which includes the free-living Paratrimastix, has a different pathway for iron-sulfur cluster synthesis that appears to run in the cytosol.

"We argued at the time that this pathway exchange is related to the loss of mitochondria in oxymonads. However, we were still interested in how it is in Paratrimastix, because although it has undergone this exchange, it still has mitochondria, so we don't really know how this organelle is beneficial to the cell," says Vladimír Hampl, explaining the background of the new study.

From previous studies, it was clear that the mitochondria of Paratrimastix pyriformis contain a called the glycine cleavage system. This complex is found in a number of other normally free-living anaerobic protists. In the mitochondria of model organisms, including humans, this complex breaks down the to form NADH, and downstream reactions produce methylated folic acid and , which are subsequently used in the cytosol for various reactions requiring a methyl group. However, it has not yet been understood what the glycine cleavage system is used for in reduced mitochondria.

Justyna Zitek and her team used an advanced proteomic method called LOPIT (Localization of Organelle Proteins by Isotope Tagging), where the cells of an organism are lysed and then several fractions are prepared by differential centrifugation. Researchers prepared nine different pellets—sedimented cell parts. "The that sediment quickly will be represented more in the first fractions," explains Vladimír Hampl.

The team then labeled these fractions with isotope tags, mixed them together and measured the mixture in a mass spectrometer. In this way, they identified over five thousand proteins and assigned many of them to . "The trick of the method is that proteins in the same compartment will sediment together and therefore their representation will be similar across fractions; we call it a profile. Because for some proteins we know in advance which compartment they belong to, we know what compartment profile looks like and can match other proteins with it," adds Hampl. Using multivariate and advanced classification methods, the team tried to assign proteins to specific compartments in the cell according to their profiles.

The main output of the study is a description of the proteome of the mitochondria of the species Paratrimastix pyriformis, to which has assigned approximately thirty proteins. From this, the researchers were able to deduce that the glycine cleavage system is followed by a sequence of three further reactions, resembling the situation in human mitochondria, which generate further derivatives from the initial product until finally the final product, formic acid, is formed.

Hampl adds, "It turns out that the reduced mitochondria obviously have an essential function for the organism, which is not the synthesis of iron-sulfur cofactors, but the synthesis of methylated compounds for the cytosol." Nothing similar has been known for simple mitochondria of anaerobic protists.

This work is also important from a methodological point of view, as it shows that the LOPIT technique can be used to study non-model organisms cultured with a mixture of bacteria that are otherwise quite difficult to characterize biochemically or proteomically.

"Our study is the second work where the LOPIT method has been applied in full scale to protozoa. It is a very elegant procedure and it was nice to show that it can be applied even to a relatively dirty culture of an organism that we would otherwise find very difficult to study," concludes Vladimír Hampl.

More information: Justyna Zítek et al, Reduced mitochondria provide an essential function for the cytosolic methionine cycle, Current Biology (2022). DOI: 10.1016/j.cub.2022.10.028

Journal information: Current Biology

Provided by Charles University

Citation: What purpose do reduced mitochondria serve? (2022, November 8) retrieved 4 February 2023 from https://phys.org/news/2022-11-purpose-mitochondria.html
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