Revealing atomistic structures behind AFM imaging

Revealing atomistic structures behind AFM imaging
Figure. 1 Illustration of the method to reconstruct the 3D atomistic protein structure from an experimental AFM surface scan. Credit: Kanazawa University

Atomic force microscopy (AFM) allows us to visualize the dynamics of single biomolecules during their functional activity. All observations are, however, restricted to regions accessible by a fairly big probing tip during scanning. Hence, AFM images only the biomolecular surface with limited spatial resolution, missing important information required for a detailed understanding of the observed phenomena.

To facilitate the interpretation of experimental imaging, Romain Amyot and Holger Flechsig from NanoLSI have now developed the mathematical framework and to reconstruct 3D atomistic structures from AFM surface scans. In their recent research paper published in PLOS Computational Biology, which is a with experimental researchers, they provide applications for high-speed AFM images ranging from single molecular machines and to large-scale assemblies of protein lattices, and they demonstrate how the full atomistic information advances the molecular understanding beyond topographic images.

The developed methods employ simulation AFM, which was previously established by Amyot and Flechsig, and distributed within the free BioAFMviewer software package (PLoS Comput Biol, 2020). Simulation AFM computationally emulates experimental scanning of biomolecules to translate structural data into simulation AFM topographic images that can be compared to real AFM images. A procedure of automatized fitting was then implemented to identify the high-resolution molecular structure behind a limited-resolution experimental AFM image. It is therefore possible to retrieve full 3D atomistic information from just a scan of the protein surface obtained under AFM .

Revealing atomistic structures behind AFM imaging
Figure. 2 From the membrane channel lattice HS-AFM image (left), the optimal-match simulation-AFM lattice (middle) provides the atomic-resolution arrangement of proteins (right). This information allows a molecular-level explanation of how these protein channels become activated. Credit: Kanazawa University

To illustrate the explanatory potential of this achievement, Flechsig says, "Imagine that instead of just seeing the tip of an iceberg, you are now able to see everything hidden under the sea, to the extent that you can even detect impurities or density differences within its structure, helping you to explain the icebergs' coloration."

To share the developments with the broad Bio-AFM community, all computational methods are embedded within the user-friendly BioAFMviewer interactive software interface. The new methods are already applied in numerous interdisciplinary collaborations to help understand experimental observations.

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BioAFMviewer software for simulated atomic force microscopy of biomolecules

More information: Romain Amyot et al, Simulation atomic force microscopy for atomic reconstruction of biomolecular structures from resolution-limited experimental images, PLOS Computational Biology (2022). DOI: 10.1371/journal.pcbi.1009970
Journal information: PLoS Computational Biology

Citation: Revealing atomistic structures behind AFM imaging (2022, July 6) retrieved 18 August 2022 from
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