Researchers elucidate the mechanics of cellular attachment

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The pictured cell originated from a malignant brain tumour. Upon removal of PPM1F, the cell can no longer detach from the ECM. It shows numerous matrix contacts, which contain the active, extended integrin carabiner (coloured in the yellow-green dots and the continuous ring). Because of the strong adhesion to the extracellular matrix, this cell can hardly move. Credit: Tanja Grimm, University of Konstanz

For cells to assemble into tissues and whole organs, the extracellular matrix (ECM) as well as integrins are required. The ECM forms a kind of extra-cellular protein meshwork; the integrins are surface proteins, which cells use to attach to this extracellular support structure. How human cells balance attachment to versus detachment from the ECM is an unresolved question. The research team led by Professor Christof Hauck in the Department of Biology at the University of Konstanz, have now identified a key enzyme called PPM1F that regulates integrin detachment from the ECM. The results have been published in the online edition of the Journal of Cell Biology.

The ECM mainly consists of a network of fibers such as collagen and other filamentous extracellular proteins. In order to adhere to this meshwork, nearly every human cell possesses integrins. Integrins operate like molecular carabiners that lock the to the network of collagen fibers or other ECM proteins and thus provide a strong focal point of attachment for the cell. However, cells do not always stay in place, sometimes migrating over long distances to their final destination—just think of that have to travel from the lymph node to a skin wound. As a solution, nature provided integrins with special features.

Like a sailor in the ship's rigging

Integrins are peculiar, because they can be repeatedly folded over and extended: When folded over, integrins cannot connect to the ECM, as the carabiner is buried. Upon extension of the , the carabiner is exposed and can lock the cell to the ECM. Interestingly, cells can extend integrins at the front end of the cell, while they fold over their integrins at the rear and detach from the ECM at these positions. Cycles of integrin-mediated attaching and detaching allow cells to move in the protein meshwork of the like a sailor climbs in the ship's rigging.

Integrins consist of two parts, the α and the β subunits. Both subunits traverse the cell membrane, so that a small part of the protein is inside the cell, but the larger portion, the actual carabiner, is outside of the cell. It has been known for some time that the extension of the integrin is initiated by the integrin β subunit: In particular, the protein talin initially binds to the intracellular part of the β subunit and triggers integrin extension and thus the activation of the carabiner.

Without phosphorylation the carabiner hook remains buried

Tanja Grimm and Nina Dierdorf, doctoral researchers at the Konstanz Research School Chemical Biology have discovered that the β subunit is being marked for talin binding by a small chemical modification, a so-called . This phos-phorylation works like a switch: Upon phosphorylation, talin can bind and the integrin is extended. If phosphorylation does not take place or if this position in the integrin is mutated, talin does not associate and the carabiner remains buried. Consequently, the cells lose their grip.

Moreover, the doctoral researchers report for the first time that a single enzyme is responsible for reversing the phosphorylation of the integrin β subunit: the protein phosphatase PPM1F. This enzyme can remove the phosphorylation and thus trigger the integrins to fold over. The PPM1F-regulated "phosphorylation switch" in the integrin seems to be essential, because in the absence of PPM1F, embryonic development terminates prematurely when different cell types have to arrange themselves into functioning tissues. Isolated cells, in which the PPM1F gene is disrupted, show enhanced attachment to the extracellular matrix and can hardly move, as they are unable to release integrin-based matrix contacts.

The researchers now hope that this knowledge can be used in the future to specifically control PPM1F activity and thus the functionality of integrins. In some tumor cells, this phosphatase appears to be particularly abundant, and the resulting reduced adhesion of such tumor cells could be one of the reasons that they are able to leave the primary tumor and form metastases at distant body sites.

"In the next step, we want to learn how to manipulate the phosphorylation switch and thus the function of integrins," says Tanja Grimm, first author of the study. "We might be able to specifically influence integrin-dependent processes in our body, from immune cell movement to tumor metastasis. With these novel findings we might help our cells to firmly stay in touch with their surrounding and prevent them straying away for the worse."


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More information: Tanja M. Grimm et al, PPM1F controls integrin activity via a conserved phospho-switch, Journal of Cell Biology (2020). DOI: 10.1083/jcb.202001057
Journal information: Journal of Cell Biology

Citation: Researchers elucidate the mechanics of cellular attachment (2020, November 9) retrieved 17 January 2021 from https://phys.org/news/2020-11-elucidate-mechanics-cellular.html
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