DNA-peptide interactions create complex behaviours which may have shaped biology
DNA-protein interactions are extremely important in biology. For example, each human cell contains about 2 meters of DNA, but this is packaged into a space about 1 million times smaller. The information in this DNA allows the cell to copy itself. This extreme packaging is mainly accomplished in cells by wrapping the DNA around proteins. Thus, how DNA and proteins interact is of extreme interest to scientists trying to understand how biology organizes itself. New research by scientists at the Earth-Life Science Institute (ELSI) at Tokyo Institute of Technology and the Institut Pierre-Gilles de Gennes, ESPCI Paris, Université PSL suggests that the interactions of DNA and proteins have deep-seated propensities to form higher-ordered structures such as those that allow the extreme packaging of DNA in cells.
Modern living cells are principally composed of a few classes of large molecules. DNA gets the lion's share of attention as it is the repository of the information cells use to build themselves generation after generation. Information-rich DNA is normally present as a double-stranded helix of two polymers wrapped around each other, with much of what makes the information DNA contains obscured to the external environment because the information-bearing parts of the molecules are engaged with their complementary strand. When DNA is copied into ribonucleic acid (RNA), its strands are pulled apart to allow its more complex surfaces to interact, which enables it to be copied into single-stranded RNA polymers. These RNA polymers are finally read out by biological processes into proteins, which are polymers of a variety of amino acids with extremely complicated surface properties. Thus, DNA and RNA are somewhat predictable in terms of their chemical behavior as polymers, while proteins are not.
Polymeric molecules, those composed or repeated types of subunits, can display complex behaviors when mixed with other chemicals, especially when dissolved in a solvent-like water. Chemists have developed a complex set of terms for how compounds behave when they are mixed. For example, the proteins in cow's milk are considered a colloidal suspension in water (a colloidal is a homogeneous noncrystalline suspended mixture that does not settle and cannot be separated by physical means). When lemon juice is added to milk, the suspended proteins reorganize themselves to produce the visible self-organization of curds, which do separate into a new phase.
There are other examples of this phenomenon chemists have discovered over the years, for example, liquid crystals. LCs are formed when molecules have an elongated shape or the tendency to make linear aggregates, like stacks of molecules one on top of each other: The resulting material presents a mixture of the properties of a crystal and a liquid—the material thus has a certain degree of order like a solid, for example, parallel orientation of the molecules, but still retains its fluidity—molecules can easily slip on and by each other. We all experience liquid crystal displays exploit these variable properties to create images.
In their work, Fraccia and Jia showed that double-stranded DNA and peptides can generate many different LC phases in a peculiar way: The LCs actually form in membraneless droplets called coacervates, where DNA and peptides are spontaneously co-assembled and ordered. This process brings DNA and peptides to very high concentrations comparable to that of a cell's nucleus, which is 100 to 1000 times greater than that of the diluted initial solution (which is the maximum concentration that can likely be achieved on early Earth). Thus, such spontaneous behavior can, in principle, favor the formation of the first cell-like structures on early Earth, which would take advantage of the ordered but fluid LC matrix in order to gain stability and functionality and to favor the growth and the evolution of primitive biomolecules.
The cut-off between when these higher-order properties begin to present themselves is not always clear. When molecules interact at the molecular level, they often "self-organize." Think of the process of adding sand to a sandpile: as more sand accumulates, it tends to form a "low energy" final state—a pile. Though the addition of sand grains may cause some new structures to form locally, at some point, the addition of one more grain causes a landslide in the pile which reinforces the conical shape of the pile.
The scientific community may be missing important aspects of the implications of this type of self-organization, Jia and Fraccia argue. The combination of these collective material self-organizing effects may be relevant at many scales of biology and may be important for biomolecular structure transitions in cell physiology and disease. In particular, the researchers discovered that various liquid crystalline structures could be accessed continuously simply by changes in environmental conditions, even as simple as changes in salinity or temperature; given the numerous unexplored conditions, this work suggests many more novel self-organized LC mesophases with potential biological function could be discovered in the near future.
This new understanding of biopolymeric self-organization may also be important for understanding how life self-organized to become living in the first place. Understanding how primitive collections of molecules could have structured themselves into collectively behaving aggregates is a significant avenue of future research.
"When the general public hears about liquid crystals, they might think of TV screens and engineering applications. However, very few would immediately think of basic science. Most researchers would not even make the connection between LCs and the origins of life. We hope this work will help increase the public's understanding of LCs in the context of the origins of life," says co-author Jia.
Finally, this work may also be relevant to disease. For example, recent discoveries regarding diseases including Alzheimer's, Parkinson's, Huntington's and ALS have pointed to intracellular phase transitions and separation leading to membraneless droplets as potential major causes.
The researchers noted that although their work was heavily impacted by the pandemic, they did their best to keep working under the global shutdowns and travel restrictions.
More information: Tommaso P. Fraccia et al, Liquid Crystal Coacervates Composed of Short Double-Stranded DNA and Cationic Peptides, ACS Nano (2020). DOI: 10.1021/acsnano.0c05083
Journal information: ACS Nano
Provided by Tokyo Institute of Technology