Plant scientists study the interaction of heat stress responses in corn

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Environmental extremes driven by climate change create stresses in crops, and plant breeders are attempting to untangle the genetic factors that endow plants with tolerance to stress. A new study from Iowa State University scientists shows how two seemingly unrelated responses in corn plants interact to help the crop survive heat stress.

The study, published on Tuesday in the The Plant Cell, shows how a called the unfolded protein response helps to activate the heat shock response when corn are exposed to hot weather conditions. The two responses operate in different parts of plant cells, and scientists previously assumed the responses were independent. But data gathered using the Enviratron, a highly controlled and automated facility at Iowa State equipped with a robotic rover and growth chambers, allowed the research team to show how one response influences another.

"These two systems have been thought to operate independently," said Stephen Howell, Distinguished Professor of Genetics, Development and Cell Biology and senior author of the study. "We've been able to show these systems sometimes work together to mitigate damage caused by heat and to protect the plant from ."

Heat stress causes proteins to denature and misfold in the endoplasmic reticulum, an organelle inside cells. Misfolded proteins can be toxic, and their buildup sets off an alarm that activates the expression of genes that protects plants from . A similar response plays out in different locations of the cell, including the cytoplasm, where excessive heat activates the expression of a different set of genes encoding heat-shock proteins.

The new study shows that, although the two responses take place in different parts of the cell, they actually work in concert during heat stress: a powerful transcription factor produced in the unfolded protein response activates the expression of a key factor helping to trigger the heat shock response.

The robotic rover visits growth chambers throughout the Enviratron facility to gather data on plants at various stages of development. Data from the Enviratron made possible a new study on heat stress in corn plants. Credit: Lie Tang

The scientists found that knocking out the made corn plants more susceptible to heat stress and hindered the heat shock response. That raises the question if overexpressing the misfolded protein response could strengthen the ability of corn plants to withstand high , but Howell said doing so may have other undesirable consequences.

"There's a seesaw balance, if you will, between defense and growth," he said. "The more you contribute to defense, the more you sacrifice growth. It may be that you could provide somewhat greater defense to crops but you might do so at the expense of growth."

In their study, the researchers drew on data gathered in the Enviratron, a state-of-the-art facility at the ISU Ag Engineering/Agronomy Research Farm that utilizes a robotic rover that travels through a series of specialized growth chambers that carefully control the environments in which the plants are raised. Development of the Enviratron was funded through a grant from the National Science Foundation. Zhaoxia Li, first author of the paper and a postdoctoral scientist in Howell's lab, said the facility allows researchers to control variables such as temperature, moisture, light and carbon dioxide concentrations to study their effect on plant development.

Howell said previous scientific papers have described the design and construction of the Enviratron, but this is the first publication in a journal based on data generated in the facility.

"We hope that studies like this will emphasize the value of conducting such research under controlled environmental conditions offered by the Enviratron," he said.

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More information: Zhaoxia Li et al. The Transcription Factor bZIP60 Links the Unfolded Protein Response (UPR) to the Heat Stress Response (HSR) in Maize, The Plant Cell (2020). DOI: 10.1105/tpc.20.00260
Journal information: Plant Cell

Citation: Plant scientists study the interaction of heat stress responses in corn (2020, August 27) retrieved 1 December 2020 from
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