New candidate for raw material synthesis through gene transfer

New Candidate for Raw Material Synthesis Through Gene Transfer
Tilman Lamparter is Professor of General Botany at the KIT. Credit: Amadeus Bramsiepe, KIT

Cyanobacteria hardly need any nutrients and use the energy of sunlight. Bathers are familiar with these microorganisms—often incorrectly called "blue-green algae"—as they often occur in waters. A group of researchers at the Karlsruhe Institute of Technology (KIT) has discovered that the multicellular species Phormidium lacuna can be genetically modified by natural transformation and could thus produce substances such as ethanol or hydrogen.

During , a cell is genetically modified by adding (DNA). This process, which occurs frequently in nature, can be used to introduce specific DNA into a cell and endow it with a certain property. "Natural transformation means that DNA is taken up by cells without any further aids," says Professor Tilman Lamparter, professor at the Botanical Institute—General Botany research field at the KIT. The procedure is simple: It works without conjugation—the connection with another cell—and without electroperforation—which would make the cell wall permeable. Since natural transformation has so far only been successful in unicellular , it was assumed that it was an exclusive feature of unicellular species. The findings of the KIT research group show that the natural competence to take up extracellular DNA occurs more frequently in cyanobacteria than previously thought. In the online scientific publication PLOS ONE, they report for the first time on gene transfer for the Phormidium lacuna genus and on the natural transformation of a multicellular, filamentous cyanobacterium.

Contribution to Bio-Economy: Replacing Fossil Resources

For natural transformation, the cells must be in a physiological state, known as natural competence, so that the recipient cell can actively transport DNA into the cytoplasm. The scientists took advantage of the natural transformation and integrated new genetic information into the genome of Phormidium lacuna. The multicellular cyanobacteria, which obtain their energy from sunlight, offer the advantage of forming a biofilm and of growing in a high cell density that can be quickly removed. KIT scientists isolated several strains of this filamentously growing species from the North Sea and the Mediterranean Sea and sequenced the genome of one strain.

New Candidate for Raw Material Synthesis Through Gene Transfer
Credit: Karlsruhe Institute of Technology

The technique established by the researchers to modify multicellular cyanobacteria by introducing genetic information opens up a wide range of possibilities for basic research and . "With the help of natural transformation, we have already created numerous so-called knockout mutants, i.e. we succeeded in switching off certain genes and thus identified their function," says Lamparter. A possible future-oriented application would be to synthesize ethanol, hydrogen or lactate as well as other bioproducts in the and thus contribute to the bio-economy and to the change from an oil-based economy to a market economy based on sustainable resources. "Our vision is to use this technology to replace fossil resources," says the biologist.

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More information: Fabian Nies et al. Natural transformation of the filamentous cyanobacterium Phormidium lacuna, PLOS ONE (2020). DOI: 10.1371/journal.pone.0234440
Journal information: PLoS ONE

Citation: New candidate for raw material synthesis through gene transfer (2020, July 2) retrieved 26 September 2020 from
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