Novel bacterial acid tolerance system sheds light on development of antimicrobials

Novel bacterial acid tolerance system sheds light on development of antimicrobials
E. coli acid tolerance system regulated by two-component system CpxRA. Credit: ZHAO Mohan

Growth ability at acidic conditions is important to bacteria. Enteric bacteria such as Escherichia coli and Salmonella can colonize and cause disease in the host's intestinal tract, but they have to combat acidic environments during the whole process of invading the host.

The stomach, with pH value as low as 1.5 to 2.5, is recognized as a natural antibiotic barrier. After entering into the , E. coli will encounter a less acidic environment (with pH value of 4-6), reproduce rapidly, and ultimately cause disease to the host.

Recently, a research team led by Prof. Xian Mo and Prof. Zhao Guang from the Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT) of the Chinese Academy of Sciences (CAS) discovered a novel bacterial acid tolerance system that confers the growth capability to E. coli at pH of 4.2.

Up to now, five acid resistance systems have been reported. These acid resistance systems can enable E. coli to survive in for hours, but they play no role in rapid multiplication of E. coli under moderate .

CpxA, the newly discovered system, can sense acidification directly through protonation of histidine residues. It will also activate its cognate regulator protein CpxRx to stimulate the expression of fabA and fabB genes for biosynthesis of unsaturated fatty acids, increasing unsaturated fatty acid contents in membrane lipid. Changes in membrane lipid composition lower the fluidity and proton permeability of the cell membrane, increasing the intracellular pH homeostasis.

Experimental results showed that E. coli mutants deficient in this system cannot grow in mouse intestine. Besides E. coli, this acid tolerance system also exists in pathogenic bacteria including Salmonella, Shigella, Vibrio cholerae, Yersinia pestis and Pseudomonas aeruginosa, which may be a new target for the development of antimicrobials.

The related findings were published in Nature Communications on Mar. 20.

More information: Ying Xu et al. An acid-tolerance response system protecting exponentially growing Escherichia coli, Nature Communications (2020). DOI: 10.1038/s41467-020-15350-5

Journal information: Nature Communications

Citation: Novel bacterial acid tolerance system sheds light on development of antimicrobials (2020, March 20) retrieved 27 November 2022 from
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