How oxygen destroys the core of important enzymes

How oxygen destroys the core of important enzymes
Hydrogenases are very sensitive to oxygen. Thus, experiments with the enzymes have to take place in a sealed environment. Credit: RUB, Marquard

New findings are expected to help protect the hydrogen-producing enzymes from harmful oxygen—which is interesting for biotechnological applications.

Certain enzymes, such as hydrogen-producing hydrogenases, are unstable in the presence of . Researchers at Ruhr-Universität Bochum (RUB) have identified the reasons on the atomic level. They outline their results in the Journal of the American Chemical Society (JACS), published online on 14 October 2019.

The experiments were carried out jointly by three RUB groups: the photobiotechnology research group was represented by Dr. Julian Esselborn—today at the University of California, San Diego –, Professor Thomas Happe and Dr. Leonie Kertess. The team collaborated with Professor Eckhard Hofmann from the Protein Crystallography Group and Dr. Ulf-Peter Apfel from the Chair of Inorganic Chemistry I.

The interdisciplinary cooperation at the interface between biology, chemistry and physics was embedded in the Ruhr Explores Solvation Cluster of Excellence, Resolv for short, and the Research Training Group Microbial Substrate Conversion, Micon for short.

Structural changes due to oxygen

The researchers analyzed a hydrogenase from the bacterium Clostridium pasteurianum. The unique aspect about this class of enzymes is that their structure consists of six iron and six sulfur atoms. The so-called cofactor constitutes the core of the protein, where the actual hydrogen production takes place.

How oxygen destroys the core of important enzymes
Since Julian Esselborn is working at the University of California, the Bochum researchers Eckhard Hofmann (left ) und Thomas Happe (right) exchange information with him via video chat. Credit: Privat

The researchers stored several samples of the enzyme with oxygen for different lengths of time. They then used X-ray structure analysis to study how the three-dimensional structure of the proteins had changed. "This method is very complex and complicated, but it helped us trace the destructive process on an ," says Julian Esselborn.

Incubation with oxygen only altered individual atoms of the , namely certain iron atoms of the cofactor. This gradually led to the disintegration of the entire active center. By understanding which iron atoms are particularly affected, the researchers hope to be able to better protect biotechnologically interesting proteins against oxygen in future.

Explore further

How nature builds hydrogen-producing enzymes

More information: Julian Esselborn et al. Loss of Specific Active-Site Iron Atoms in Oxygen-Exposed [FeFe]-Hydrogenase Determined by Detailed X-ray Structure Analyses, Journal of the American Chemical Society (2019). DOI: 10.1021/jacs.9b07808
Citation: How oxygen destroys the core of important enzymes (2019, November 4) retrieved 1 December 2020 from
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