A molecular hammock for cotranslational modification

A molecular hammock for cotranslational modification
3-D reconstruction of the ribosome-N-acetyltransferase complex, based on an analysis of cryo-electron micrographs. Credit: Roland Beckmann, LMU

Proteins do most of the real work in cells and are modified in accordance with functional requirements. An LMU team has now shown how proteins are chemically altered on the ribosome, even before they fold into the active conformations.

The functional properties of proteins – whether they serve as catalytic enzymes or play purely structural roles – are largely dictated by their three-dimensional shapes, which are in turn dependent on their amino- sequences and can be altered by chemical modification. LMU structural biologist Professor Roland Beckmann studies the many essential tasks performed by proteins in cells, focusing in particular on those that take place during their synthesis. Protein synthesis is carried out by molecular machines known as ribosomes, which are themselves composed of proteins and ribosomal RNAs. These 'organelles' translate genetic blueprints encoded in 'messenger RNAs' into the specific sequences of that define the particular function of each type of in cells. Ribosomes work rather like assembly lines, connecting amino acids together in the sequence dictated by the mRNA to which they are bound. Beckmann and his research group at LMU's Gene Center have now used to determine how certain enzymes interact with ribosomes to chemically modify nascent proteins. By imaging purified and flash-frozen ribosomal complexes in the electron microscope, they were able to reconstruct the modification process in three dimensions. The study appears in the journal Nature Structural and Molecular Biology.

Coordination of cotranslational modifications

Many proteins undergo chemical modification while they are still associated with the . Protein synthesis begins at the N-terminal end and, as the chain of amino acids grows, it is directed into the exit tunnel of the ribosome. In the cells of higher organisms, the first amino acid in most proteins is modified by the attachment of an acetyl group (CH3COO) by enzymes known as N-acetyltransferases (NATs). This reaction takes place 'cotranslationally' as the N-terminal amino acid emerges from the tunnel, while the rest of the protein is still being synthesized. The NAT-dependent modification is essential for the cellular functions of the proteins. "However, the nature of the interaction between these acetyltransferases and the ribosome was not known prior to our study," says co-author Dr. Birgitta Beatrix.

With the aid of their 3-D reconstruction of the NAT-ribosome complex, she and her colleagues were able to show that the enzyme is positioned at the exit of the ribosomal tunnel, where it "can swing back and forth like a hammock," as first author Alexandra Knorr puts it. This is made possible by the fact that it can attach to four binding patches on the ribosomal RNA. Moreover, three of these binding sites are located in what are known as expansion segments, which act as flexible spacers and are specific for the rRNA of eukaryotic organisms (whose cells are nucleated).

"What these segments do was hitherto enigmatic, and our results have now assigned a function to them," says Beatrix. Because the expansion segments stick out above the surface of the ribosome, they create a gap between the tunnel exit and the catalytic center of the acetyltransferase. This gap between the exit and the must be bridged by a stretch of at least 20 amino acids to enable the N-terminal to occur. As a result, other modifying enzymes can gain access to these amino acids before the acetyltransferase has a chance to act. "These results have allowed us, for the first time, to propose a mechanistic model of how the actions of different cellular components are coordinated in order to cotranslationally modify still nascent proteins," says Beckmann.


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More information: Alexandra G. Knorr et al. Ribosome–NatA architecture reveals that rRNA expansion segments coordinate N-terminal acetylation, Nature Structural & Molecular Biology (2018). DOI: 10.1038/s41594-018-0165-y
Citation: A molecular hammock for cotranslational modification (2018, December 20) retrieved 24 June 2019 from https://phys.org/news/2018-12-molecular-hammock-cotranslational-modification.html
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