A detailed look at the microorganisms that colonize, and degrade, a 400-year-old painting

December 5, 2018, Public Library of Science
Carlo Bononi, Incoronazione della Vergine: 1616-1620, oil on canvas, 280 cm in diameter, Ferrara, Basilica of Santa Maria in Vado: a) painting (sampling points are indicated by asterisks, *); b) damage due to biodegradation (verso); c) craquelures network; d) lacerations, deformations, and gaps corresponding to the cloth stitching (recto). Details of the damage are reported on the side. Reprinted from http://www.museoinvita.it/lincoronazione-della-vergine-di-carlo-bononi/ under a CC BY license, with permission from "MuseoinVita" biannual journal, original copyright 2018. Credit: CC BY license, with permission from "MuseoinVita" biannual journal, original copyright 2018

What's a feast for the human eye may be a literal feast for microorganisms that colonize works of art, according to a new study in the open-access journal PLOS ONE by Elisabetta Caselli of the University of Ferrara, Italy, and colleagues. The researchers characterized the microbial community on a 17th century painting and showed that while some microbes destroy such works of art, others might be employed to protect them.

The wide variety of organic and inorganic materials that comprise a , such as canvas, oil, pigments, and varnish, can provide an ideal environment for colonizing and fungi, increasing the risk for biodegradation. To characterize the microorganisms on one such painting, "Incoronazione della Virgine" by Carlo Bononi, completed in 1620, the authors removed a 4 mm2 section of the painted surface adjacent to a damaged area.

Using a combination of microscopy and microbial culture techniques, the authors identified a variety of microbes which had colonized the painting. They isolated multiple strains of Staphylococcus and Bacillus bacteria as well as filamentous fungi of the Aspergillus, Penicillium, Cladosporium, and Alternaria genera. The authors note that some of the 17th century paint pigments used, notably red lac and red and yellow earths, may be nutrient sources for the microbes. They also tested a decontaminating biocompound which contained spores of three Bacillus bacteria and found that these could inhibit growth of both the bacteria and the fungi isolated from the painting.

Bacteria detected on the painting: Samples were collected from the recto (a, b, c) and the verso (d, e, f) of the painting. a) colonies of Staphylococcus spp. on a Mego agar plate; b) the same Staphylococcus spp. viewed by OM after Gram staining (original magnification 100X) and c) by SEM. d) colonies of Bacillus spp. on a TSA agar plate; e) the same Bacillus spp. viewed by OM after Gram staining (original magnification 100X) and f) SEM. Credit: Caselli et al., 2018

The authors conclude that a wide range of bacterial and may inhabit such ancient paintings, but biocompounds potentially represent a novel approach for preserving works of art at risk of biodegradation.

The authors add: "Clarification of biotederioration processes in artworks is important, as it could help in preventing or solving the associated damages. This study investigated such aspects in a 17th century painting, by analyzing both and chemical composition of painting, also evaluating a possible biological way to counteract these phenomena."

Explore further: Plants rely on their resident bacteria to protect them from harmful microbes

More information: Caselli E, Pancaldi S, Baldisserotto C, Petrucci F, Impallaria A, Volpe L, et al. (2018) Characterization of biodegradation in a 17th century easel painting and potential for a biological approach. PLoS ONE 13(12): e0207630. doi.org/10.1371/journal.pone.0207630

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