The exocyst dynamo

cells
Credit: CC0 Public Domain

The exocyst is a protein complex essential for life, that is comprised of eight subunits and is a crucial component in vesicle trafficking.

The mechanisms by which exocysts assemble and deliver vesicles containing important biological materials to the has been unclear, especially in . Much of this is because previous studies using gene overexpression can produce artifacts.

Now, using CRISPR/Cas9-mediated gene editing, high-speed single molecule sensitive imaging and fluorescence cross-correlation spectroscopy, Mukhtar Ahmed, Ph.D., and colleagues have found that the mammalian exocyst functions as a remarkably dynamic two-part complex, thus providing a new mechanism of how the exocyst functions.

The researchers also discovered that the exocyst subcomplexes, each composed of four subunits, assemble and localize to the plasma membrane independently of each other. The study was published last week in the journal Nature Communications.

The authors said that the methods they employed are applicable to any protein complexes in the cell and have the potential to revolutionize our understanding of cell dynamics.


Explore further

Research describes missing step in how cells move their cargo

More information: Syed Mukhtar Ahmed et al. Exocyst dynamics during vesicle tethering and fusion, Nature Communications (2018). DOI: 10.1038/s41467-018-07467-5
Journal information: Nature Communications

Citation: The exocyst dynamo (2018, December 17) retrieved 25 May 2019 from https://phys.org/news/2018-12-exocyst-dynamo.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
4 shares

Feedback to editors

User comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more