Fully identified—the pathway of protons

Fully identified—the pathway of protons
Martin Winkler, Jifu Duan und Thomas Happe (from the left) traced the pathway of protons all the way into the active center of hydrogenases. Credit: RUB, Marquard

In their catalytic center, hydrogenases manufacture molecular hydrogen (H2) from two protons and two electrons. They extract the protons required for this process from the surrounding water and transfer them – via a transport chain – into their catalytic core. The exact proton pathway through the hydrogenase had as yet not been understood. "This transfer pathway is a jigsaw piece, crucial for understanding the interplay of cofactor and protein which is the reason why biocatalysts are so much more efficient than hydrogen-producing chemical complexes," explains Dr. Martin Winkler, one of the authors of this study from the Photobiotechnology research group at RUB.

In order to figure out which of the hydrogenase building blocks are involved in , the researchers substituted them individually. They replaced each either by an amino acid with a similar function or by a dysfunctional amino . Thus, 22 variants of two different hydrogenases were created. Subsequently, the researchers compared those variants with regard to different aspects, including their spectroscopic properties and their enzyme activity. "The molecular structures of twelve protein variants, which were solved using X-ray structure analysis, proved particularly informative," says Winkler.

Amino acids with no function shut down hydrogenases

Depending on where and how the researchers had changed the hydrogenase, hydrogen production became less efficient or stopped altogether. "Thus, we ascertained why some variants are severely impaired in terms of and why others are hardly impaired at all – against all expectations," says Martin Winkler.

The closer to the catalytic centre the replaced amino acids were located, the less able the hydrogenase was to compensate for these modifications. If building blocks with no function were embedded in sensitive locations, was shut down. "The thus generated state resembles an oversaturation due to stress where protons as well as hydrogen are simultaneously introduced into the ," elaborates Martin Winkler. "In the course of our project, we were for the first time able to stabilise and analyse this highly transient state that we had already encountered in experiments."

Valuable baseline information

This study has made it possible to assign the functions of individual amino acids to the proton transfer pathway for the enzyme group of [FeFe] hydrogenases. "Moreover, it provides valuable information on the molecular mechanism of proton transfer by redox-active proteins and the structural requirements thereof," concludes Thomas Happe.


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More information: Jifu Duan et al. Crystallographic and spectroscopic assignment of the proton transfer pathway in [FeFe]-hydrogenases, Nature Communications (2018). DOI: 10.1038/s41467-018-07140-x
Journal information: Nature Communications

Citation: Fully identified—the pathway of protons (2018, November 9) retrieved 23 September 2019 from https://phys.org/news/2018-11-fully-identifiedthe-pathway-protons.html
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