Gene network lets plant roots handle nitrogen

Gene network lets plant roots handle nitrogen
Nitrogen metabolism is vitally important to plants. The Arabidopsis plant on right was grown in low-nitrogen conditions, meaning it had to grow longer roots to find more nitrogen. Researchers at UC Davis and the Cold Spring Harbor Laboratory have now identified a complex of genes involved in harvesting and processing nitrogen in plants. Credit: Allison Gaudinier, UC Davis

With robotics, computers and advanced genetics, researchers at the University of California, Davis and Cold Spring Harbor Laboratory are unraveling how plant roots take up and metabolize nitrogen, the key to plant growth and crop yield. Their latest work is published Oct. 24 in the journal Nature.

"Nitrogen metabolism is incredibly important for growth," said Siobhan Brady, associate professor of plant biology at UC Davis and senior author on the paper. The invention of over a hundred years ago has enabled a massive expansion in agricultural productivity to feed billions of people. But at the same time, runoff of excess pesticides into soils, waterways and the oceans has many negative impacts.

By understanding the that control how take up and use , scientists like Brady hope to give plant breeders tools to generate crop varieties that need less fertilizer or make better use of it.

"We know the genes that are involved in nitrogen assimilation and transport but we don't understand all the ways that nitrogen metabolism is regulated," Brady said.

What's more, most of these regulatory genes, called transcription factors because they control the transcription (or activity) of other genes, have been identified in stems, shoots and leaves—but not many in roots, where nitrogen actually gets into a plant from the soil.

Science at the root

Brady's laboratory aims to discover the networks of genes that shape how live and grow. Because nitrogen is so important to plants, graduate student Allison Gaudinier and Brady took the premise that transcription factors for nitrogen metabolism would also be linked to other important processes.

New regulators of nitrogen use in plants identified
Leaf from transgenic (top) and non-transgenic (bottom) maize plants, collected from same node, grown in low nitrogen field. Credit: DuPont Pioneer

Gaudinier used robotics to screen against hundreds of genes at a time, assembling them into a network. Adjunct Associate Professor Doreen Ware and colleagues at Cold Spring Harbor Laboratory used computational methods to predict which genes were most important in the network. The UC Davis team could then study the role of those genes in plants.

The results establish a core set of genes that are critical in nitrogen metabolism, Brady said. "If we want to breed nitrogen-efficient plants, we need to look at these genes," she said. "This will open up a lot of research."

More information: Allison Gaudinier et al, Transcriptional regulation of nitrogen-associated metabolism and growth, Nature (2018). DOI: 10.1038/s41586-018-0656-3

Journal information: Nature

Provided by UC Davis

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