Seeing pesticides spread through insect bodies
Now a team at Osaka University has developed a new method of visualizing the behavior of pesticides inside insect bodies. Their findings were recently published in Analytical Sciences and highlighted on the journal's cover.
Lead author Seitaro Ohtsu explains, "There have been no reports on the distribution of agricultural chemicals in insects to date. This is probably because it's very difficult to prepare tissue sections of Drosophilia specimens for imaging studies."
Researchers from Osaka University examined an insect from the Drosophila family, a type of fruit fly widely used for testing pesticides. They developed a technique to slice the insect body into thin sections for analysis while preserving the delicate structures of the specimen.
Imidacloprid—a highly effective nicotine-related pesticide—was chosen for the analysis. Applying their sample preparation method to insects treated with this chemical allowed the team to follow its uptake, breakdown and distribution in the insects' bodies.
The team applied a method that involves scanning a laser across the thin sections of the insect body to eject material from small areas of the surface. By analyzing the chemical composition of the ejected material with a mass spectrometer at different locations, they were able to build up a picture of the pesticide and its breakdown products over the whole insect body.
Senior researcher Shuichi Shimma says, "This is a timely contribution while the evidence for the negative effects of certain pesticides on ecosystems is accumulating. We hope our technique will help other researchers gain new insights into pesticide metabolism that might help limit the effects of pesticides to their targets without harming beneficial pollinating insects."