A promising new tool to measure antibodies against malaria

July 4, 2018, Barcelona Institute for Global Health
A multipipette is used for handling multiple samples when performing quantitative suspension arrays. Credit: Pau Fabregat

Antibodies against multiple Plasmodium falciparum proteins (or antigens) can be measured using a simple, accurate and reproducible assay that requires very small amounts of blood. In a series of recently published articles, a team led by ISGlobal reports the development and optimisation of several quantitative suspension array assays (qSATs) to assess natural and vaccine-induced responses to malaria and other parasites.

Antibody responses against P. falciparum are critical in controlling or preventing malaria. However, relatively little is known about the type and specificity of these protective , or how they work. This is further complicated by the fact that the parasite expresses more than 5000 proteins.

Carlota Dobaño and her group have developed new laboratory protocols to measure antibodies to multiple P. falciparum antigens in one single reaction and from small amounts of circulating blood. The qSAT used here consists of coupling small microspheres or beads to different parasite antigens and then testing if they are recognized by plasma samples of individuals exposed (or not) to malaria. The authors first adapted the assay to detect different types and classes of antibodies against multiple P. falciparum antigens in a sensitive and specific manner. Second, they showed that this simple multiplex assay is highly reproducible not only between experiments but also between operators and laboratories. Third, they identified which are the key factors that need to be optimised to reduce variability of the assay. Finally, they used this test to better characterise the antibody profile of the anti-malaria human plasma pool provided by the WHO as reference reagent. They showed that it contains low levels of antibodies to CSP (the antigen contained in the RTS,S vaccine), suggesting that customized reference pools may be needed for studying responses to certain antigens.

These simple and reproducible multiplex protocols will allow us to analyse in detail natural and vaccine-induced antibody responses to large panels of P. falciparum antigens, and elucidate correlates of malaria protection," explains Dobaño. "Furthermore, the assay is highly versatile and can be adapted to study responses to antigens from other microbes or vaccines. We are currently collaborating with several groups outside the field," she adds.

Explore further: New strategy for vaccinating pregnant mothers against malaria holds promise for protecting infants

More information: Itziar Ubillos et al, Analysis of factors affecting the variability of a quantitative suspension bead array assay measuring IgG to multiple Plasmodium antigens, PLOS ONE (2018). DOI: 10.1371/journal.pone.0199278

Related Stories

Improving human immunity to malaria

August 1, 2012

The deadliest form of malaria is caused the protozoan Plasmodium falciparum. During its life-cycle in human blood, the parasite P. falciparum expresses unique proteins on the surface on infected blood cells.

Discovery finds possible new route to malaria vaccine

December 28, 2015

Oxford University researchers across the globe are working to beat Malaria. Now, a team of Oxford scientists in the UK and Kenya, working with colleagues in three Swiss institutes, have found two people who could reveal a ...

Recommended for you

The powerful meteor that no one saw (except satellites)

March 19, 2019

At precisely 11:48 am on December 18, 2018, a large space rock heading straight for Earth at a speed of 19 miles per second exploded into a vast ball of fire as it entered the atmosphere, 15.9 miles above the Bering Sea.

OSIRIS-REx reveals asteroid Bennu has big surprises

March 19, 2019

A NASA spacecraft that will return a sample of a near-Earth asteroid named Bennu to Earth in 2023 made the first-ever close-up observations of particle plumes erupting from an asteroid's surface. Bennu also revealed itself ...

Nanoscale Lamb wave-driven motors in nonliquid environments

March 19, 2019

Light driven movement is challenging in nonliquid environments as micro-sized objects can experience strong dry adhesion to contact surfaces and resist movement. In a recent study, Jinsheng Lu and co-workers at the College ...

Revealing the rules behind virus scaffold construction

March 19, 2019

A team of researchers including Northwestern Engineering faculty has expanded the understanding of how virus shells self-assemble, an important step toward developing techniques that use viruses as vehicles to deliver targeted ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.