Tuneable genetic 'clocks' might lead to improved biotech strategies

May 2, 2018 by Caroline Brogan, Imperial College London
Credit: Imperial College London

Imperial scientists have worked out how to fine-tune cellular clocks, which might lead to optimised production of drugs, biofuels and other chemicals.

In genetics, oscillators are biological 'clocks' that express certain key genes at regular time intervals. Such oscillators exist in all realms of life, from bacteria to humans—and their rhythms are at the core of essential processes like cell division and metabolism.

Oscillators can be manipulated by external cues. For example, the day/night cycle influences the genes that the release of melatonin, increasing its levels at night to induce and maintain sleepiness.

Scientists are increasingly giving genetic oscillators to engineered cells, to help them control the release of drugs, biofuels, and other chemicals. Yet until now it has been difficult to control the and of oscillations separately.

Now, a study in Cell Systems by researchers at Imperial College London has shown new ways of better controlling genetic oscillations by tuning the desired amplitude and frequency.

This could let cells 'budget' resources by reducing the amount of time that genes stay 'on', which cuts the energy burden and the accumulation of toxic compounds in the cell. This would streamline the process of producing drugs, biofuels, and other chemicals, making operations more productive and efficient.

Lead author Dr. Guy-Bart Stan, from Imperial's Department of Bioengineering, said: "We have shown that it's possible to independently tune the amplitude and frequency of genetic clocks, giving us greater control over their output."

Unravelling rhythms

Currently, host bacterial are genetically altered to express genes that control the expression of desired proteins. Genetic oscillators were some of the first bio-circuits constructed in synthetic biology and have already been used in various applications, but until now scientists have been unable to change the amplitude without affecting the frequency, or vice versa.

The researchers used computer modelling to unravel the links between amplitude and frequency in current genetic oscillators, and, based on this, proposed new design principles to control amplitude and frequency independently.

These newly designed genetic clocks could help build more complex genetic networks for delivery, and even introduce a personalised medicine element.

First author Dr. Marios Tomazou, also from the Department of Bioengineering, said: "Our findings could help us make drugs that take effect at specific times depending on benefit to specific patients. This approach could make drugs even more effective, while reducing any side effects caused by being always 'on'."

Next, the researchers will take the concept from computer models to laboratory tests to test whether it works in a practical setting.

Explore further: E. coli rewired to control growth as experts let them make proteins for medicine

More information: Nicholas A. Rossi et al. Making Waves with Synthetic Oscillators, Cell Systems (2018). DOI: 10.1016/j.cels.2018.04.001

Marios Tomazou et al. Computational Re-design of Synthetic Genetic Oscillators for Independent Amplitude and Frequency Modulation, Cell Systems (2018). DOI: 10.1016/j.cels.2018.03.013

Related Stories

Researchers create focused spin wave beams

December 22, 2015

Researchers at the University of Gothenburg Physics Department have finally found the secret to synchronize an unlimited number of spintronic oscillators. Such devices are very promising for future applications requiring ...

Recommended for you

Houseplants could one day monitor home health

July 20, 2018

In a perspective published in the July 20 issue of Science, Neal Stewart and his University of Tennessee coauthors explore the future of houseplants as aesthetically pleasing and functional sirens of home health.

Putting bacteria to work

July 20, 2018

The idea of bacteria as diverse, complex perceptive entities that can hunt prey in packs, remember past experiences and interact with the moods and perceptions of their human hosts sounds like the plot of some low-budget ...

LC10 – the neuron that tracks fruit flies

July 20, 2018

Many animals rely on vision to detect, locate, and track moving objects. Male Drosophila fruit flies primarily use visual cues to stay close to a female and to direct their courtship song towards her. Scientists from the ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.