Antibiotics may reduce the ability of immune cells to kill bacteria

November 30, 2017, Broad Institute of MIT and Harvard
Mitochondria. Credit: Wikipedia commons

Antibiotics normally act in concert with an organism's immune system to eliminate an infection. However, the drugs can have broad side effects, including eliminating "good" bacteria in the course of fighting off a pathogen. A new study led by researchers from the Broad Institute of MIT and Harvard, MIT, and the Wyss Institute for Biologically Inspired Engineering has shown that antibiotics can also reduce the ability of mouse immune cells to kill bacteria, and that changes to the biochemical environment directly elicited by treatment can protect the bacterial pathogen. The work was published today in Cell Host & Microbe.

"Antibiotics interact with , particularly , in ways we didn't expect," said co-first author Jason Yang, a postdoctoral scholar at the Broad Institute and MIT. "And the biochemical context, altered by and cells in the surrounding tissue, matters when you're trying to predict how a drug might work in different people or in different infections."

In the face of the growing threat of antibiotic resistance, understanding the myriad impacts of antibiotics is also a critical goal for researchers and clinicians formulating better treatments. Senior author James Collins—an institute member at Broad, professor at MIT, and core faculty member at the Wyss Institute—and his lab have previously shown that several types of antibiotics can damage mitochondria in mice and in human epithelial cells, and that bacterial susceptibility to drugs can be affected by small molecules, called metabolites, released by cells as intermediates of their metabolic reactions.

Yang and his colleagues suspected that might further alter the infection microenvironment in ways that impact bacteria and immune cells. To investigate, the team treated mice infected by Escherichia coli bacteria with a commonly used antibiotic called ciprofloxacin, administered through the animals' drinking water at concentrations relative to what a human would receive, and quantified the biochemical changes.

The researchers found that the antibiotic treatment elicited systemic changes in metabolites—not by influencing the microbiome, but by acting directly on the mouse tissues. On further investigation, the team determined that metabolites released by made E. coli more resistant to ciprofloxacin. Antibiotic exposure also impaired immune function by inhibiting respiratory activity in immune cells: Macrophages treated with ciprofloxacin were less able to engulf and kill E. coli bacteria.

"You generally assume that antibiotics will significantly impact the , and yet here they seem to be triggering responses in mammalian cells," said Collins. "The drugs are producing changes that are actually counterproductive to the treatment effort. They reduce the bacterial susceptibility to antibiotics, and the drugs themselves reduce the functional benefit of the immune cells."

The results highlight the potential of antibiotics to modulate the immune system, and reveal the importance of the metabolic microenvironment in resolving an infection. "Aspects of this work indicate that biochemical diversity could be a driver of different drug treatment outcomes," said Yang. "If we have a better understanding of the specific effects that antibiotics can have on different cells, that may help us make decisions about how to better treat infection."

Translating the findings to human health will require more follow-up work. "We need to do additional animal studies under a broader range of conditions with a broader range of antibiotics, and potentially measure metabolites in human patients undergoing treatment, to see what else might be happening," said Collins.

Explore further: New way to test antibiotics could lead to better drugs

More information: Yang JH, Bhargava P, et al. Antibiotic-induced changes to the host metabolic environment inhibit drug efficacy and alter immune function. Cell Host & Microbe. Online November 30, 2017. DOI: 10.1016/j.chom.2017.10.020

Related Stories

New way to test antibiotics could lead to better drugs

September 1, 2017

MIT and Harvard University researchers have engineered E. coli cells that can be used to study how bacteria at an infection site respond to antibiotic treatment, allowing scientists to learn more about how existing antibiotics ...

Major breakthrough in fighting antibiotic resistance

February 29, 2016

In what is being heralded as a groundbreaking discovery, scientists led by Monash and Walter and Eliza Hall Institute researcher, Thomas Naderer and James Vince, have shown that drugs, originally developed to kill cancer ...

Recommended for you

EPA adviser is promoting harmful ideas, scientists say

March 22, 2019

The Trump administration's reliance on industry-funded environmental specialists is again coming under fire, this time by researchers who say that Louis Anthony "Tony" Cox Jr., who leads a key Environmental Protection Agency ...

Coffee-based colloids for direct solar absorption

March 22, 2019

Solar energy is one of the most promising resources to help reduce fossil fuel consumption and mitigate greenhouse gas emissions to power a sustainable future. Devices presently in use to convert solar energy into thermal ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.