In-cell NMR: A new application

In-cell NMR: A new application
(a) Proteins (green) can be endogenously expressed and isotopically labelled in bacteria (b) Exogenous proteins (blue) can be delivered to X. Credit: Enrico Luchinat and Lucia Banci

The structure of biological macromolecules is critical to understanding their function, mode of interaction and relationship with their neighbours, and how physiological processes are altered by mutations or changes in the molecular environment.

Ideally, classical structural biology research should interface more with , as it is crucial for the structural data obtained in vitro to be validated within the cellular or tissue context. A true cellular structural biology approach should allow macromolecules to be characterised directly in their native environment. Such an approach would guarantee the high significance of data obtained in vivo or in the cell with the high resolution of a structural technique.

In the Past decade, NMR spectroscopy has been applied to obtain structural and functional information on inside intact, living cells. The approach, termed "in-cell NMR", utilises the improved resolution and sensitivity of modern high-field NMR spectrometers and exploits selective enrichment of the molecule(s) of interest with NMR-active isotopes.

Since its inception, in-cell NMR has gradually emerged as a possible link between structural and cellular approaches. Being especially suited to investigate the structure and dynamics of macromolecules at atomic resolution, in-cell NMR can fill a critical gap between in vitro-oriented structural techniques such as NMR spectroscopy, X-ray crystallography and single-particle cryo-EM techniques and ultrahigh-resolution cellular imaging techniques, such as cryo-electron tomography.

In a topical review IUCrJ (2017), 4, 108-118 Lucia Banci and her co-worker Enrico Luchinat , both based at the University of Florence, summarise the major advances of in-cell NMR and report the recent developments in the field, with particular focus on its application for studying proteins in eukaryotic and mammalian cells and on the development of cellular solid-state NMR.


Explore further

Catching a glimpse at enzymes on the job

More information: Enrico Luchinat et al, In-cell NMR: a topical review, IUCrJ (2017). DOI: 10.1107/S2052252516020625
Citation: In-cell NMR: A new application (2017, March 8) retrieved 21 August 2019 from https://phys.org/news/2017-03-in-cell-nmr-application.html
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
2 shares

Feedback to editors

User comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more