How a germ catches a virus

June 30, 2016

Bacteriophages – short phages – represent a group of small viruses that infect bacteria and are able to alter or destroy them. That is why their name can be translated as bacteria-eater. In order to infect a bacterium, a phage has to first recognize structures on the bacterial cell wall and adhere to it. Under the leadership of Professor Thilo Stehle, Cengiz Koç and other coworkers of the Collaborative Research Center 766 (The Bacterial Cell Envelope) at the University of Tübingen have investigated this mechanism of recognition. They focused their studies on the pathogenic bacterium Staphylococcus aureus and the phage Phi11, which infects staphylococci. The scientists identified a phage protein that mediates the recognition of the receptor on the bacterium. Details on the three-dimensional structure of this protein and its mode of recognition of the bacterial receptor were elucidated with the help of X-ray structure analysis.

The scientists published their work in two recent articles of the journal Scientific Reports. The results could contribute to the development of a novel therapy against bacterial infections.

Phages insert into the genes of bacteria and "hide" there until they reactivate under certain conditions to initiate the destruction of the cell. Bacteria are by far the most abundant life form on earth, and yet there still exist about ten times more than bacteria. Phages exert a selection pressure and contribute substantially to the evolution of bacteria. "Among other things, they are complicit in the generation of novel, sometimes even highly infectious bacterial species," says Thilo Stehle. On the other hand, bacteria are also capable to exploit phage components by using their protein structures for other purposes. In daily life, phages are especially useful in the food industry, in the health care system, and for analytical diagnostics.

In order to find out by what mechanism bacteriophage phi11 adheres to the of staphylococci, the scientists used a combination of bioinformatical, microbiological and structure analytical methods. The characterization of the receptor-binding protein (Rbp) was initially probed through immunogold-labeled specific antibodies. The interactions on the phages were then analyzed by electron microscopy and through infection studies. These data demonstrated that an essential chemically altered building unit in the cell wall scaffold is crucial for the interaction with the receptor-binding protein.

The X-ray structure analysis of the receptor-binding protein, which was carried out in Tübingen, revealed the Rbp to be a complex, elongated molecule consisting of three subunits. "In addition to the actual receptor-binding component, the protein also possesses an unusual hinge region which could work as a tilting mechanism," explains Stehle. "This mechanism could allow the phage to bind to receptors coming from different orientations." Furthermore the Rbp contains an enzymatically active element, which apparently is able to digest sugar structures on the host organism's surface in order to obtain access to the actual receptors. The scientists expect that the underlying mechanism of receptor recognition is similar for many sugar-binding phages. "Our studies also contribute to a better understanding of evolutionary processes involving phages," the scientist concludes.

Explore further: How viruses infect bacteria: A tale of a tail

More information: Xuehua Li et al. An essential role for the baseplate protein Gp45 in phage adsorption to Staphylococcus aureus, Scientific Reports (2016). DOI: 10.1038/srep26455

Cengiz Koç et al. Structure of the host-recognition device of Staphylococcus aureus phage ϕ11, Scientific Reports (2016). DOI: 10.1038/srep27581

Related Stories

How viruses infect bacteria: A tale of a tail

May 18, 2016

Bacteriophages are viruses that infect bacteria. Using state-of-the-art tools, EPFL scientists have described a million-atom "tail" that bacteriophages use to breach bacterial surfaces. The breakthrough has major implications ...

Scientists bolster 'phage' weapons in food safety battle

July 22, 2015

In the war to keep food safe from bacteria, Cornell food scientists examine a class of weaponry called bacteriophages – an all-natural biological enemy for the nasty Listeria monocytogenes, which threatens meat, produce, ...

Researchers Examine How Viruses Destroy Bacteria

November 18, 2009

Viruses are well known for attacking humans and animals, but some viruses instead attack bacteria. Texas A&M University researchers are exploring how hungry viruses, armed with transformer-like weapons, attack bacteria, which ...

Recommended for you

Novel framework to infer microbial interactions

December 11, 2017

Inferring the underlying ecological networks of microbial communities is important to understanding their structure and responses to external stimuli. But it can be very challenging to make accurate network inferences. In ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.