Quenching mechanism of 2D MnO2 nanosheet towards Au nanocluster fluorescence clarified

April 15, 2016, Nanjing University

Researchers at Nanjing University have now clarified the quenching mechanism of 2D MnO2 nanosheets on Au nanocluster fluorescence and then built a turn on fluorescent sensor for sensitive and selective detection of glutathione (GSH).

Ultra-thin MnO2 nanosheets, one of the promising2D nanomaterials, are of particular interests and have found wide applications in bioanalytical and biomedical studies due to such features as their unique photophysical property, rich redox chemistry, and good biocompatibility. Owing to their broad absorption from d-d transition of Mn2+, MnO2 nanosheets act as effective quenchers toward numerous reporters. By combining such interesting photophysical properties with redox chemistry, biosensing and bioimaging have been achieved with MnO2 nanosheets. However, the quenching mechanism of fluorescent reporters by MnO2 nanosheets still remains largely elusive, which in turn would impede their wide application.

To address these issues, Wei and co-workers adopted a nanocluster fluorescent reporter, which is made of a few to hundreds of metal atoms and is more stable than organic dyes. They prepared protein-stabilized Au nanoclusters (named as AuNC), and systematically investigated their fluorescent quenching behaviors using MnO2 nanosheets. Interestingly, both dynamic and static quenching effects played critical roles in the quenching process while fluorescent resonance energy transfer (FRET) and inner filter effect (IFE) only played very minor roles in the quenching process. Moreover, they developed a sensitive and selective turn-on sensor for fluorescent detection of GSH, an important antioxidant involved in many cellular processes and diseases. With their newly established method, highly sensitive and selective detection of GSH has been achieved.

Their study further illuminates the mechanisms of nanomaterials-induced fluorescence quenching. It also paves a way to designing turn-on fluorescent sensors for bioanalysis and bioimaging.

Explore further: Team develops novel eco high performance energy storage device

More information: 10.1039/C6AY00199H Shichao Lin et al. Deciphering the quenching mechanism of 2D MnO nanosheet towards Au nanocluster fluorescence to design effective glutathione biosensor , Anal. Methods (2016). DOI: 10.1039/C6AY00199H

Related Stories

Recommended for you

Atomic-scale ping-pong

June 20, 2018

New experiments by researchers at the National Graphene Institute at the University of Manchester have shed more light on the gas flow through tiny, angstrom-sized channels with atomically flat walls.

Chameleon-inspired nanolaser changes colors

June 20, 2018

As a chameleon shifts its color from turquoise to pink to orange to green, nature's design principles are at play. Complex nano-mechanics are quietly and effortlessly working to camouflage the lizard's skin to match its environment.

Method could help boost large scale production of graphene

June 19, 2018

The measure by which any conductor is judged is how easily, and speedily, electrons can move through it. On this point, graphene is one of the most promising materials for a breathtaking array of applications. However, its ...


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.