Cell-based tests promise respite for lab animals: study

January 26, 2016
Using human cells in Petri dishes in the lab, scientists tested about 10,000 different types of chemical compounds including pes
Using human cells in Petri dishes in the lab, scientists tested about 10,000 different types of chemical compounds including pesticides, industrial chemicals, food additives and drugs

Scientists in the United States said Tuesday they were developing a faster, more efficient way of gauging the toxicity of chemicals, which may reduce the need for animal testing.

Using human cells in Petri dishes in the lab, they tested about 10,000 different types of chemical compounds including pesticides, industrial chemicals, food additives and drugs.

The results were used to build models to "predict" whether the compounds, or combinations of them, may be harmful to humans or the environment when used in or environmental chemicals.

Toxicity is one of the main reasons that new drugs fail, and it is hoped this library of toxicity data may spot unsafe chemical compounds at a far earlier phase of research.

"Thousands of chemicals to which humans are exposed have inadequate data on which to predict their potential for toxicological effects," the study authors wrote in the journal Nature Communications.

Traditional toxicity tests using animals are expensive and species differences mean they do not always accurately predict a chemical's effect on humans.

They also raises ethical concerns about animal welfare.

The project, dubbed Tox21, is a joint effort involving three US federal agencies: the Environmental Protection Agency, the National Institutes of Health (NIH) and the Food and Drug Administration (FDA).

"An important goal of the US Tox21 program is to use in vitro (Petri dish) data as surrogates for in vivo (live) toxicity to reduce animal testing," study co-author Ruili Huang of the NIH told AFP by email.

But to achieve this, cell tests have to be just as predictive, or more so, of as animal tests.

After testing about 10,000 compounds, each in 15 different concentrations and on different cells, the team used their database to build predictive models for new chemical combinations.

They found that their models could predict for both humans and animals, and may be "a promising alternative to traditional animal toxicology studies."

Further work must be done to validate and improve the models, which Huang said may never completely replace animal testing.

It may, however, allow scientists to prioritise chemicals predicted to be most likely toxic for further tests using traditional methods, and so "significantly reduce the amount of required," he said.

Animal activists have welcomed the research.

"To understand more about human development and human diseases, the world's most forward-thinking scientists are developing and implementing methods that supersede the crude use of animals," Julia Baines, science policy advisor of the organisation People for the Ethical Treatment of Animals (PETA) told AFP.

"PETA welcomes the wealth of advanced non-animal research methodologies that are creating a brighter future for and human health."

Explore further: A cell imaging-based screening approach predicts toxic effects of chemicals on the human kidney

More information: Modelling the Tox21 10 K chemical profiles for in vivo toxicity prediction and mechanism characterization, Nature Communications 7, Article number: 10425 DOI: 10.1038/ncomms10425

Related Stories

US Tox21 to begin screening 10,000 chemicals

December 7, 2011

A high-speed robotic screening system, aimed at protecting human health by improving how chemicals are tested in the United States, begins today to test 10,000 compounds for potential toxicity. The compounds cover a wide ...

Recommended for you

Commercially relevant bismuth-based thin film processing

September 20, 2018

Developing materials suitable for use in optoelectronic devices is currently a very active area of research. The search for materials for use in photoelectric conversion elements has to be carried out in parallel with developing ...

A game of pool in the live cell

September 20, 2018

Cells need to react to environmental changes and maintain a balanced system of signaling cascades within the cell. Proteins outside of the cell, on the cellular surface, inside the cellular membrane, and within the cell orchestrate ...

Nucleation a boon to sustainable nanomanufacturing

September 19, 2018

Calcium carbonate is found nearly everywhere, in sidewalk cement, wall paint, antacid tablets and deep underground. Engineers at Washington University in St. Louis have used a unique set of state-of-the-art imaging techniques ...

Greater than the sum of its parts

September 18, 2018

When it comes to designing and optimizing mechanical systems, scientists understand the physical laws surrounding them well enough to create computer models that can predict their properties and behavior. However, scientists ...


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.