Some plants regenerate by duplicating their DNA

Some plants regenerate by duplicating their DNA
Some cultivars of the plants Arabidopsis thaliana (used in the study) and Ipomopsis aggregata (pictured here) can duplicate their genomes multiple times without undergoing cell division. Credit: L. Brian Stauffer

When munched by grazing animals (or mauled by scientists in the lab), some herbaceous plants overcompensate - producing more plant matter and becoming more fertile than they otherwise would. Scientists say they now know how these plants accomplish this feat of regeneration.

They report their findings in the journal Molecular Ecology.

Their study is the first to show that a plant's ability to dramatically rebound after being cut down relies on a process called genome duplication, in which individual cells make multiple copies of all of their genetic content.

Genome duplication is not new to science; researchers have known about the phenomenon for decades. But few have pondered its purpose, said University of Illinois animal biology professor Ken Paige, who conducted the study with postdoctoral researcher Daniel Scholes.

"Most - 90 percent - duplicate their genomes," Paige said. "We wanted to know what this process was for."

In a 2011 study, Paige and Scholes demonstrated that that engage in rampant genome duplication also rebound more vigorously after being damaged. The researchers suspected that genome duplication was giving the plants the boost they needed to overcome adversity.

That study and the new one focused on Arabidopsis thaliana, a plant in the mustard family that often is used as a laboratory subject. Some Arabidopsis plants engage in and others don't. Those that do can accumulate dozens of copies of all of their chromosomes in individual cells.

In the new study, Scholes crossed Arabidopsis plants that had the ability to duplicate their genomes with those that lacked this ability. If the relationship between DNA duplication and regeneration was mere happenstance, the association between the two should disappear in their offspring, Scholes said.

Some plants regenerate by duplicating their DNA
Animal biology professor Ken Paige (left) and postdoctoral fellow Daniel Scholes found that a plant's ability to duplicate its genome within individual cells influences its ability to regenerate. Credit: L. Brian Stauffer

"But the association persisted in the offspring," he said. "That's the first line of evidence that these two traits seem to be influencing each other."

To further test the hypothesis, Scholes experimentally enhanced an Arabidopsis plant's ability to duplicate its genome. He chose a line that lacked that ability and that also experienced a major reduction in fertility after being grazed.

As expected, the altered plant gained the ability to vigorously rebound after being damaged, the researchers reported.

"We were able to completely mitigate the otherwise detrimental effects of damage," Scholes said. "There was no difference in fertility between damaged and undamaged plants."

Genome duplication enlarges cells and provides more copies of individual genes, likely increasing the production of key proteins and other molecules that drive cell growth, Scholes said. Future studies will test these ideas, he said.


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Some plants duplicate their DNA to overcome adversity

More information: "Plasticity in ploidy underlies plant fitness compensation to herbivore damage," onlinelibrary.wiley.com/doi/10 … 1/mec.12894/abstract
Journal information: Molecular Ecology

Citation: Some plants regenerate by duplicating their DNA (2014, November 11) retrieved 18 November 2019 from https://phys.org/news/2014-11-regenerate-duplicating-dna.html
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