Food allergies: A new, simple method to track down allergens

Although food allergies are common, sufferers often don't know exactly what in foods cause their allergic reactions. This knowledge could help develop customized therapies, like training the body's immune system to respond to certain proteins found in foods. However, determining which protein in a food causes an allergic response to a patient requires time-consuming tests that often ignore rare or unexpected allergens. Publishing in Analytical Chemistry, EPFL scientists have developed a highly-sensitive method that can quickly and accurately identify the culprit proteins even at very low concentrations. The method has been successfully tested in the context of cow milk allergy.

Food allergies are becoming widespread in the Western world today, affecting around 6-8% of children and about 3% of adults. These types of allergies occur when the body's immune system mistakes a harmless food protein for a threat and attacks it as it would normally do with a bacterium or a virus. This causes symptoms like swelling, rashes, pain, and even life-threatening anaphylactic shocks.

Cow is common among children, preventing them from breast feeding and drinking milk, although some outgrow the allergy by six years of age. Allergies, including food allergies, are caused when our produces antibodies to destroy "enemy" molecules, like those from bacteria and viruses. In the case of milk allergies, the antibodies are called "IgE". Medical doctors can diagnose milk allergies by simply detecting an overproduction of IgE, but that does not tell them which one of the numerous proteins in milk – and other foods – is causing the .

The team of Hubert Girault at EPFL has developed a highly-sensitive method that uses a patient's IgE to determine specifically which protein induces allergic responses in them. The method uses a well-established technique called immunoaffinity capillary electrophoresis (IACE). First, IgE antibodies from the patient's blood are isolated by interaction with magnetic beads that are coated with a different type of antibody. The "bead" antibodies recognize and bind the patient's IgE antibodies. This takes place inside a long and narrow glass tube, only 50 micrometers in diameter, called a "capillary". The bound antibodies are then flushed out of the capillary and powerfully attached to the magnetic beads through a process called 'crosslinking', which keeps them from detaching. The beads with the patient's IgE are then placed again inside the capillary.

The test begins when milk is injected through the capillary. As the milk's proteins pass over the patient's IgE antibodies, the ones that cause allergies are caught by them, while the others exit on the other side. The beads are then washed with a strong chemical that causes the allergy-inducing protein to dissociate from the patient's IgE antibodies. The isolated, "" protein is then identified using mass spectrometry, which is a technique that analyzes compounds according to their mass and electrical charge.

The method offers a personalized way to identify the exact proteins that can cause allergies to a patient, which can help develop an effective treatment. It is also quicker, as it does not require the detection and quantification of a patient's specific IgE or the laborious and resource-intensive diagnostic methods used currently. Finally, it provides higher accuracy than conventional allergy-testing methods, as it can detect tiny amounts of allergic proteins, even if they are unexpected and rare. This also means that the method can be extended beyond milk to other foods like nuts and wheat products.

More information: Gasilova N, Girault HH. Component-Resolved Diagnostic of Cow's Milk Allergy by Immunoaffinity Capillary Electrophoresis–Matrix Assisted Laser Desorption/Ionization Mass Spectrometry. Analytical Chemistry 10 June 2014. DOI: 10.1021/ac500525n

Journal information: Analytical Chemistry

Citation: Food allergies: A new, simple method to track down allergens (2014, July 2) retrieved 28 March 2024 from https://phys.org/news/2014-07-food-allergies-simple-method-track.html
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