Protein anchors help keep embryonic development 'just right'
The "Goldilocks effect" in fruit fly embryos may be more intricate than previously thought. It's been known that specific proteins, called histones, must exist within a certain range—if there are too few, a fruit fly's DNA is damaged; if there are too many, the cell dies. Now research out of the University of Rochester shows that different types of histone proteins also need to exist in specific proportions. The work further shows that cellular storage facilities keep over-produced histones in reserve until they are needed.
The findings were published today in the journal Current Biology.
Welte had previously discovered that another protein—called Jabba—anchored histones onto lipid droplets. In his latest research, he found that excess histones migrate outside the nucleus to the droplets, where they are temporarily held until needed to create new chromosomes.
"People have observed histone proteins on lipid droplets in multiple organisms, including mammals," said Welte. "The results of this research project may very well help us understand the role of histones and lipid droplets in humans."
Welte found that by deleting the Jabba anchors from the cell, one particular type of histone increased in proportion in the nucleus, since they had no way to be held in reserve away from the cell nuclei. That made the embryo more sensitive to environmental stresses like higher temperature, leading to defects during cell division and reduced viability.
Just as it is in humans, the embryonic stage is a crucial time for the fruit fly (Drosophila melanogaster). Starting from a single cell, it has to rapidly multiply in cell number and develop into a larva while coping with stresses from the environment. The embryo does all of that by activating a myriad of genetic on-off switches, a process that involves unwrapping and rewrapping various regions of DNA.
Histones are important to the process because they act as spools that DNA molecules wrap around to form chromosomes, making it possible for the DNA to do its work in the first place. Welte discovered that if the different histones are not in the correct proportion, the embryo has trouble developing correctly and may even die.
Achieving the correct proportion of histones is not something that happens automatically in the fruit fly embryo, as Welte found in his research. Instead, when a certain histone type is made in excess, it is redirected to the lipid-droplet holding sites outside the nucleus where it is kept until needed.
Welte and his team will try next to identify which parts of the Jabba protein actually bind with the histone. Once that's determined, scientists may have the ability to manipulate the histone storage process.
"Since histones and lipid droplets are found in humans, I very much expect that there will be a similar storage process," said Welte. "If that is the case, our findings could one day help treat or prevent diseases linked to chromosome malfunction. What I find most fascinating is that our research uncovered this intimate link between cellular fat depots and the nucleus, a connection that just a few years ago nobody would have dreamt of. "