'Electronic' DNA sequencing: Changes in molecular charge act as signal

March 14, 2006

Sometimes it is only individual base pairs that differentiate a diseased gene from a healthy one (point mutations). The systematic exploration of such variations, as well as the diagnosis of genetic diseases, require the simplest, most cost-effective and rapid sequencing methods possible.

Japanese researchers have now developed a new approach for a miniaturized “electronic” process that detects small differences in DNA sequences with high sensitivity. In contrast to prior methods, this technique works without labeling of the bases. It is based on a special semiconducting element called a field effect transistor (FET), which detects changes in the charges on DNA molecules.

The sequencing method developed by Toshiya Sakata and Yuji Miyahara uses a chip with multiple FETs. An FET is a semiconductor component that “feels” electrical fields on its surface. It reacts to changes in the field with a change in the current that flows through its conducting channel. The researchers loaded the surface of such an FET with short, single-stranded pieces of DNA. These probes are the exact counterparts to the sequence at the beginning of the DNA segment being investigated. If a sample containing the target DNA comes into contact with the surface, the target DNA binds to the probes.

The enzyme polymerase could then be used to complete the probe to a complete counterpart of the target DNA, if the four necessary building blocks— the nucleosides adenosine, cytidine, thymidine, and guanosine (A, C, T, and G)—were present in the solution. This is where the special trick comes in: the chip is dipped alternately into four different solutions, each containing only one of the building blocks. After each dip, the electrical characteristics of the FET are measured.

If and only if a component has been added to the end of the chain, a change is registered. This occurs because each building block brings with it a negative charge, which changes the electrical field on the surface of the FET. In this way, DNA chains of a length up to about ten components can be precisely sequenced. Missing, extra, or changed nucleotides can be rapidly and unambiguously identified.

As electrical signals are measured, this method can easily be standardized. Miniature arrays containing multiple FETs with different probes allow for the parallel analysis of different gene segments.

Source: Angewandte Chemie

Explore further: Biosensor could help diagnose illnesses directly in serum

Related Stories

Biosensor could help diagnose illnesses directly in serum

August 30, 2017

In this age of fast fashion and fast food, people want things immediately. The same holds true when they get sick and want to know what's wrong. But performing rapid, accurate diagnostics on a serum sample without complex ...

Recommended for you

Metacognition training boosts gen chem exam scores

October 20, 2017

It's a lesson in scholastic humility: You waltz into an exam, confident that you've got a good enough grip on the class material to swing an 80 percent or so, maybe a 90 if some of the questions go your way.

Two teams independently test Tomonaga–Luttinger theory

October 20, 2017

(Phys.org)—Two teams of researchers working independently of one another have found ways to test aspects of the Tomonaga–Luttinger theory that describes interacting quantum particles in 1-D ensembles in a Tomonaga–Luttinger ...

Dawn mission extended at Ceres

October 20, 2017

NASA has authorized a second extension of the Dawn mission at Ceres, the largest object in the asteroid belt between Mars and Jupiter. During this extension, the spacecraft will descend to lower altitudes than ever before ...

0 comments

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.