Producing protein from circular RNA in E.ColiMay 22, 2013 in Chemistry / Biochemistry
Circular RNAs were recently shown to be abundant in mice and humans where they influence gene expression.
In a study published today in the journal Angewandte Chemie, the team, led by Dr Hiroshi Abe report that circular RNA strands treated with the E. coli cell-free system can be translated to produce 100 times more protein than their linear counterparts.
"The translation process is a lot more efficient on circular RNAs than on linear templates because the speed of the re-initiation process is greatly increased," explains Dr. Abe.
This new technique could be used for the synthesis of tandem-repeat peptides such as those found in biologically important proteins such as silk, collagen and epidermal growth factor.
Abe, N. et al. Rolling circle amplification in a prokaryotic translation system using small circular RNA, Angewandte Chemie International Edition, 2013
Provided by RIKEN
"Producing protein from circular RNA in E.Coli" May 22, 2013 http://phys.org/news/2013-05-protein-circular-rna-ecoli.html