Producing protein from circular RNA in E.Coli

May 22nd, 2013 in Chemistry / Biochemistry
Producing protein from circular RNA in E.Coli

Circular RNAs were recently shown to be abundant in mice and humans where they influence gene expression.

In a study published today in the journal Angewandte Chemie, the team, led by Dr Hiroshi Abe report that circular treated with the E. coli cell-free system can be translated to produce 100 times more protein than their linear counterparts.

"The translation process is a lot more efficient on circular RNAs than on linear templates because the speed of the re-initiation process is greatly increased," explains Dr. Abe.

This new technique could be used for the synthesis of tandem-repeat peptides such as those found in biologically important proteins such as silk, collagen and epidermal growth factor.

More information: Abe, N. et al. Rolling circle amplification in a prokaryotic translation system using small circular RNA, Angewandte Chemie International Edition, 2013

Provided by RIKEN

"Producing protein from circular RNA in E.Coli." May 22nd, 2013. http://phys.org/news/2013-05-protein-circular-rna-ecoli.html