Resolving the internal structure of nanoparticle dimers linked by DNA

January 16th, 2013 in Nanotechnology / Bio & Medicine
Resolving the Internal Structure of Nanoparticle Dimers Linked by DNA
Figure A: Calculated distribution of interparticle separation, r, for dimers linked by either one or four DNA chains. The average separation decreased with increasing number of particle-connecting chains.


Figure A: Calculated distribution of interparticle separation, r, for dimers linked by either one or four DNA chains. The average separation decreased with increasing number of particle-connecting chains.

Light and x-ray scattering experiments have revealed the structure of nanoparticle dimers linked by flexible DNA chains. These dimers were basic units in a multi-scale,hierarchical assembly, and served as a model system to understand DNA-mediated interactions, particularly in the non-trivial regime when the nanoparticle and DNA links were comparable in size. We found that the interparticle separation within the dimer was primarily controlled by the number of linking DNA. Researchers summarize their findings in a simple model that captured the interplay of the number of DNA bridges, their length,the curvature of the nanoparticle, and the excluded volume effects. We demonstrated excellent agreement of our analytical model with both our experimental and computational results, without use of free parameters in the model.  

As building blocks in multi-scale assemblies, DNA-linked nanoparticle dimers are excellent model systems to understand chain-mediated interactions between particles.  The results of this study can serve to guide the design of precisely controlled distances within nanoscale clusters; such control is imperative for energy transfer and bio-detection functionalities, among other applications.

Figure B: Simulation protocol for dimer formation of two DNA-coated NPs connected by four linkers. (a) Two DNA-coated NPs and four linkers (pink strands) are located at random positions. (b) Four strands of one hemisphere of each NP are randomly selected. (c) Hybridization occurs between the linkers and strands of the left NP. (d) Hybridization with second NP followed by equilibration.

Details:

More information: Cheng, C. et al. Internal Structureof Nanoparticle Dimers Linked by DNA. ACS Nano 6 (8), 6793-6802 (2012).
pubs.acs.org/doi/abs/10.1021%2Fnn301528h

Provided by Brookhaven National Laboratory

"Resolving the internal structure of nanoparticle dimers linked by DNA." January 16th, 2013. http://phys.org/news/2013-01-internal-nanoparticle-dimers-linked-dna.html