Filming life in the fast laneJune 4, 2012 in Biology / Cell & Microbiology
New microscope enabled scientists at EMBL Heidelberg to film a fruit fly embryo, in 3D, from when it was about two-and-a-half hours old until it walked away from the microscope as a larva.
"This video shows a fruit fly embryo from when it was about two-and-a-half hours old until it walked away from the microscope as a larva, 20 hours later," says Lars Hufnagel, from the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany. "It shows all the hallmarks of fruit fly embryonic development in three dimensions."
The video was obtained using a new microscope created by Hufnagel's lab and described online today in Nature Methods. In it, you can watch as cells on the embryo's belly dive in to form what's known as the ventral furrow. Other cells can then be seen moving around the embryo's rear end to its back (inset), in a process called convergent extension. And later, when an opening appears in the embryo's back (inset), see the surrounding cells close the gap in a process known as dorsal closure.
The new microscope, called Multi-View SPIM, or MuVi-SPIM for short, enables scientists to image rapid biological processes in thick samples at unprecedented detail. It builds upon the Selective-Plane Illumination Microscopy (SPIM) technology developed at EMBL a few years ago. Like SPIM, the new microscope shines a thin sheet of light on the embryo, illuminating one layer of a sample like this embryo at a time, to obtain an image of the whole sample with minimal light-induced damage. But MuVi-SPIM takes four full images from different angles, eliminating the need to rotate the sample. This speeds up the imaging and enables scientists to merge the four images into a single high-quality three-dimensional image for the first time.
Hufnagel and colleagues can obtain a high-quality three-dimensional image of a fruit fly embryo in a few seconds, and be ready to repeat the process. This means the different images that make up the video are taken in such rapid succession that very little has changed in the embryo from one frame to the next so scientists can be sure of where each cell or even structures inside cells is and track it throughout the video. Using such fast imaging, Hufnagel and colleagues were able to record the movements of every nucleus in the embryo not only during the later developmental stages shown here, but also throughout the first three hours of the embryo's life, when nuclei divide very rapidly.
"Everything in this microscope is optimised for speed and light-sensitivity," says Uros Krzic from Hufnagel's lab, who designed the microscope. "Not just the optics, mechanics and cameras, but also the software that controls the microscope and processes the data. We can finally get high-quality data in real time."
In the future, the scientists would like to use their new microscope to investigate the how organs and tissues form, not only in the fruit fly but also in other model organisms.
Provided by European Molecular Biology Laboratory
"Filming life in the fast lane" June 4, 2012 http://phys.org/news/2012-06-life-fast-lane.html