New technique lets scientists peer within nanoparticles, see atomic structure in 3-D

March 21st, 2012 By Kim DeRose in Nanotechnology / Nanophysics
Jianwei Miao and colleagues have developed an electron tomography method to image the 3-D structure of a gold nanoparticle at a resolution of 2.4 angstroms. Individual atoms are observed in some regions of the particle and several grains are identified in three dimensions. In the figure, the four three-dimensional grains (green and gold; blue and red) form two pairs of twin boundaries inside the nanoparticle. Credit: Jianwei Miao/UCLA Physics & Astronomy, CNSI


Jianwei Miao and colleagues have developed an electron tomography method to image the 3-D structure of a gold nanoparticle at a resolution of 2.4 angstroms. Individual atoms are observed in some regions of the particle and several grains are identified in three dimensions. In the figure, the four three-dimensional grains (green and gold; blue and red) form two pairs of twin boundaries inside the nanoparticle. Credit: Jianwei Miao/UCLA Physics & Astronomy, CNSI

(PhysOrg.com) -- UCLA researchers are now able to peer deep within the world's tiniest structures to create three-dimensional images of individual atoms and their positions. Their research, published March 22 in the journal Nature, presents a new method for directly measuring the atomic structure of nanomaterials.

"This is the first experiment where we can directly see local structures in three dimensions at atomic-scale resolution — that's never been done before," said Jianwei (John) Miao, a professor of physics and astronomy and a researcher with the California NanoSystems Institute (CNSI) at UCLA.

Miao and his colleagues used a scanning transmission electron microscope to sweep a narrow beam of high-energy electrons over a tiny gold particle only 10 nanometers in diameter (almost 1,000 times smaller than a red blood cell). The nanoparticle contained tens of thousands of individual gold , each about a million times smaller than the width of a human hair. These atoms interact with the electrons passing through the sample, casting shadows that hold information about the nanoparticle's interior structure onto a detector below the microscope.

Miao's team discovered that by taking measurements at 69 different angles, they could combine the data gleaned from each individual shadow into a 3-D reconstruction of the interior of the nanoparticle. Using this method, which is known as electron tomography, Miao's team was able to directly see individual atoms and how they were positioned inside the specific gold nanoparticle.

Presently, X-ray crystallography is the primary method for visualizing 3-D molecular structures at atomic resolutions. However, this method involves measuring many nearly identical samples and averaging the results. X-ray crystallography typically takes an average across trillions of molecules, which causes some information to get lost in the process, Miao said.

"It is like averaging together everyone on Earth to get an idea of what a human being looks like — you completely miss the unique characteristics of each individual," he said.

X-ray crystallography is a powerful technique for revealing the structure of perfect crystals, which are materials with an unbroken honeycomb of perfectly spaced atoms lined up as neatly as books on a shelf. Yet most structures existing in nature are non-crystalline, with structures far less ordered than their crystalline counterparts — picture a rock concert mosh pit rather than soldiers on parade.

"Our current technology is mainly based on crystal structures because we have ways to analyze them," Miao said. "But for non-crystalline structures, no direct experiments have seen atomic structures in three dimensions before."

Probing non-crystalline materials is important because even small variations in structure can greatly alter the electronic properties of a material, Miao noted. The ability to closely examine the inside of a semiconductor, for example, might reveal hidden internal flaws that could affect its performance.

"The three-dimensional atomic resolution of non-crystalline structures remains a major unresolved problem in the physical sciences," he said.

Miao and his colleagues haven't quite cracked the non-crystalline conundrum, but they have shown they can image a structure that isn't perfectly crystalline at a resolution of 2.4 angstroms (the average size of a gold atom is 2.8 angstroms). The gold nanoparticle they measured for their paper turned out to be composed of several different crystal grains, each forming a puzzle piece with atoms aligned in subtly different patterns. A nanostructure with hidden crystalline segments and boundaries inside will behave differently from one made of a single continuous crystal — but other techniques would have been unable to visualize them in , Miao said.

Miao's team also found that the small golden blob they studied was in fact shaped like a multi-faceted gem, though slightly squashed on one side from resting on a flat stage inside the gigantic microscope — another small detail that might have been averaged away when using more traditional methods.

This project was inspired by Miao's earlier research, which involved finding ways to minimize the radiation dose administered to patients during CT scans. During a scan, patients must be X-rayed at a variety of angles, and those measurements are combined to give doctors a picture of what's inside the body. Miao found a mathematically more efficient way to obtain similar high-resolution images while taking scans at fewer angles. He later realized that this discovery could benefit scientists probing the insides of nanostructures, not just doctors on the lookout for tumors or fractures.

Nanostructures, like patients, can be damaged if too many scans are administered. A constant bombardment of high-energy electrons can cause the atoms in nanoparticles to be rearranged and the particle itself to change shape. By bringing his medical discovery to his work in materials science and nanoscience, Miao was able to invent a new way to peer inside the field's tiniest structures.

The discovery made by Miao's team may lead to improvements in resolution and image quality for tomography research across many fields, including the study of biological samples.

Provided by University of California Los Angeles

"New technique lets scientists peer within nanoparticles, see atomic structure in 3-D." March 21st, 2012. http://phys.org/news/2012-03-technique-scientists-peer-nanoparticles-atomic.html