DNA sieve -- Nanoscale pores can be tiny analysis labs

May 11, 2007
DNA sieve -- Nanoscale pores can be tiny analysis labs
Graphic showing a lipid bilayer membrane (blue) with an alpha-hemolysin nanopore. A polyethylene glycol molecule (green globular structure) is transiting the pore; others are in solution on one side of the membrane. The colored spheres represent individual atoms, and are approximately 0.5 nanometers in diameter, or one twenty-thousandth the width of a human hair. Credit: NIST

Imagine being able to rapidly identify tiny biological molecules such as DNA and toxins using a system that can fit on a microchip or in a drop of salt water. It’s closer than you might think, say a team of researchers from the National Institute of Standards and Technology, Brazil’s Universidade Federal de Pernambuco, and Wright State University in Dayton, Ohio.

In a paper appearing next week in the Proceedings of the National Academy of Sciences, the team proves for the first time that a single nanometer-scale pore in a thin membrane—resembling one found in a living cell—can be used to accurately detect and sort different-sized polymer chains (a model for biological molecules) that pass through the channel.

Traditionally, unknown molecules in solution are measured and identified using mass spectrometry, a process that involves ionizing and disintegrating large numbers of the target molecule, then analyzing the masses of the resulting fragments to produce a "molecular fingerprint" for the original sample. The required equipment can cover a good-sized desk. By contrast, the "single-molecule mass spectrometry" system described in the PNAS paper is a non-destructive technique that in principle can measure one molecule at a time in a space small enough to fit on a single microchip-based device the size of a cell phone or PDA.

The technique involves creating a "lipid bilayer," a membrane of biological molecules similar to the walls of living cells, and creating a pore in it with a protein such as alpha-hemolysin, produced by the Staphyloccoccus aureus bacteria specifically to poke holes in cells. The target molecules are forced one-at-a-time through the nanopore, which is only 1.5 to 12 nanometers in diameter, by an applied electric current. As the molecules—in this experiment, different-sized chains of the polymer polyethylene glycol (PEG)—pass through the channel, the current flow is reduced in proportion to the size of each individual chain, allowing an easy measure of its mass.

As a control, a solution of a highly purified PEG of a specific size was characterized with the nanopore. The resulting "fingerprint" closely matched the one identifying samples of the same size polymer in the mixed-chain solution.

Further enhancement of the data from both the experimental and control tests yielded mass measurements and identifications of the different PEG chains that correlate with those made by traditional mass spectrometry.

Because the dimensions of the lipid bilayer and the alpha-hemolysin pore, as well as the required amount of electrical current, are at the nanoscale level, the "single-molecule mass spectrometry" technology may one day be incorporated into "lab-on-a-chip" molecular analyzers and single-strand DNA sequencers.

Citation: J.W.F. Robertson, C.G. Rodriguez, V.M. Stanford, K.A. Rubinson, O.V. Krasilnikov and J.J. Kasianowicz. Single-moelcule mass spectrometry in solution using a solitary nanopore. Proceedings of the National Academy of Sciences, 104 (20): 8207, May 15, 2007.

Source: National Institute of Standards and Technology

Explore further: Gold nanorods target cancer cells

add to favorites email to friend print save as pdf

Related Stories

NIST suggests new purity test for biotech products

Dec 03, 2014

To avoid contaminating their experiments, biomedical researchers want to know that the scientific products they buy are pure. But how pure does something need to be to really be pure? Using a new test, scientists ...

Recommended for you

Gold nanorods target cancer cells

Dec 18, 2014

Using tiny gold nanorods, researchers at Swinburne University of Technology have demonstrated a potential breakthrough in cancer therapy.

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.