Molecular motors and brakes work together in cells

Jan 30, 2007
Molecular motors and brakes work together in cells
Daughter microtubule (red) being formed on mother microtubule (red), within yeast cell (dashed line). Green represents the molecular motors, the kinesin proteins klp2, of each microtubule. Credit: Phong Tran, PhD, University of Pennsylvania School of Medicine

Researchers at the University of Pennsylvania School of Medicine have discovered that microtubules -- components responsible for shape, movement, and replication within cells -- use proteins that act as molecular motors and brakes to organize into their correct structure. If microtubules are not formed properly such basic functions as cell division and transport can go wrong, which may have implications in such disease processes as cancer and dementia.

The study, published in the January issue of Cell, is featured on the cover of that issue.

"Up until now motors and brakes were studied separately from microtubules," says senior author Phong Tran, PhD, Assistant Professor of Cell and Developmental Biology. "This study lets us have a more complete picture."

Microtubules are structures that help give shape to many types of cells, form the spindle -- a structure important in cell division -- and act as a railroad, of sorts, upon which molecular motors move protein packages for waste removal and nerve transmission.

In the Cell study, the investigators, working with fission yeast cells, showed that stable end-to-end arrays of microtubules can be achieved by a balance between the sliding by a molecular motor called klp2p and the braking of a microtubule-associated protein (MAP) called ase1p. Specifically, they showed that a preexisting "mother" microtubule acts as a platform on which a new microtubule can be formed. The new "daughter" microtubule grows and moves along the mother microtubule. In time, the daughter grows beyond the end of the mother to ultimately produce two microtubules, connected by the cross-linking MAP ase1p.

"Imagine that the daughter microtubule is a short train on the track of the mother microtubule," explains Tran. "The molecular motor is the train's engine, but the problem is that the cargo -- the molecular brakes -- gets longer, slowing down the daughter train. But when the train gets to the end of the track it remains attached to the end of mother microtubule. At the tail end, it stops moving and that defines the region of overlap. Our work shows that the cell can make microtubule structures of defined lengths stable by coordinating the sliding of the motors and the slowing of the brakes."

If microtubule-based structures are not formed properly because of failures in brakes or motors, such basic functions as cell division and cell transport can go awry, with such diseases as cancer and dementia possibly resulting. "For the first time we have shown how MAPs and motors work together in a mechanistic way," says Tran. "This is important and it will make other people who study microtubules rethink how they study the cell."

Source: University of Pennsylvania

Explore further: Ivory mafia: how criminal gangs are killing Africa's elephants

add to favorites email to friend print save as pdf

Related Stories

Revealing the inner workings of a molecular motor

Jan 12, 2015

In research published in the Journal of Cell Biology, scientists from the RIKEN Brain Science Institute in Japan have made important steps toward understanding how dynein—a "molecular motor"—walks along ...

Recommended for you

Obama recommends extended wilderness zone in Alaska

1 hour ago

US President Barack Obama said Sunday he would recommend a large swath of Alaska be designated as wilderness, the highest level of federal protection, in a move likely to anger oil proponents.

Uganda seizes massive ivory and pangolin haul

3 hours ago

Ugandan wildlife officers have seized a huge haul of elephant ivory and pangolin scales, representing the deaths of hundreds of endangered animals, police said Sunday.

The elephant poaching business in numbers

11 hours ago

From the pittance paid to local poachers to a multi-billion dollar industry, here are some of the key numbers related to Africa's endangered elephants:

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.