Starvation keeps sleep-deprived fly brain sharp

Aug 31, 2010

As anyone who has ever struggled to keep his or her eyes open after a big meal knows, eating can induce sleepiness. New research in fruit flies suggests that, conversely, being hungry may provide a way to stay awake without feeling groggy or mentally challenged.

Scientists at Washington University in St. Louis found that allows the need for nourishment to push aside the need for . Like humans and rats, cannot survive without sleep. But in a line of flies engineered to be sensitive to sleep deprivation, starvation nearly tripled the amount of time they could survive without sleep.

Researchers showed that the ability to resist the effects of was linked to a protein that helps the fruit fly brain manage its storage and use of lipids, a class of molecules that includes fats such as cholesterol and fat-soluble vitamins such as vitamins A and D.

"The major drugs we have to either put people to sleep or keep them awake are all targeted to a small number of pathways in the brain, all of them having to do with neurotransmission," says Paul Shaw, PhD, assistant professor of and anatomy. "Modifying processing with drugs may provide us with a new way of tackling that is more effective or has fewer side effects."

The study appears online Aug. 31 in PLoS Biology.

The findings add a new wrinkle to the complex relationship between sleep and dietary metabolism. Scientists recognized about a decade ago that inadequate sleep results in obesity and contributes to the development of diabetes and coronary disease. Until now, no one had connected genes linked to lipids with regulation of the need for sleep.

Clay Semenkovich, MD, a Washington University lipid expert not directly involved in the study, says the results fit into a growing awareness that organisms use lipids for much more than energy storage.

"It's becoming apparent that fats serve as signaling molecules in a number of contexts," says Semenkovich, the Herbert S. Gasser Professor of Medicine. "If you identify the appropriate lipids involved in sleep regulation and figure out how to control them, you may be able to decrease suffering associated with loss of sleep or the need to stay awake."

Shaw uses fruit flies as models for sleep's effects in higher organisms. He was among the first to prove that flies enter a state comparable to sleep, showing that they have periods of inactivity where greater stimulation is required to rouse them. Like humans, flies deprived of sleep one day will try to make up for it by sleeping more the next day, a phenomenon referred to as sleep debt. Sleep-deprived flies also perform poorly on a simple test of learning ability.

Studies in other labs have shown that starvation or, in the case of human volunteers, fasting leads to less sleep. More recent research has also shown that starvation can change the activity levels of genes that manage storage and use of lipids.

Shaw's lab previously demonstrated that fruit flies with a mutation in a timekeeping gene accumulate sleep debt much more quickly and begin dying after being kept awake for as little as 10 hours. Matt Thimgan, PhD, a postdoctoral research associate, reports in the new paper that starving fruit flies spent more time awake, and starving fruit flies with the timekeeping gene mutation could survive up to 28 hours without sleep.

Scientists tested the starving, sleepless flies for two markers of sleep debt: an enzyme in saliva and the flies' ability to learn to associate a light with an unpleasant stimulus. Both tests showed that the starving flies were not getting sleepy.

"From an evolutionary perspective, this makes sense," Thimgan says. "If you're starving, you want to make sure you're on the top of your game cognitively, to improve your chances of finding food rather than becoming food for someone else."

Scientists found an effect similar to starvation in fruit flies where a gene called Lipid storage droplet 2 (LSD2) was disabled. After sleep deprivation, flies with the LSD2 mutation were less likely to sleep for longer periods of time and continued to score high on the learning test.

"LSD2 mutants seem to constantly rotate lipids through their storage depot in cells, putting them in and moving them out very quickly," Thimgan says. "Disabling LSD2 appears to make it hard for cells to hold on to lipids and use them properly, and we think this impairs brain cells' ability to respond to sleep deprivation."

Researchers are working to identify the specific lipids affected by loss of LSD2.

Explore further: New viral tools for mapping brains

More information: Thimgan MS, Suzuki Y, Seugnet L, Gottschalk L, Shaw PJ. The Perilipin homologue, Lipid storage droplet 2, regulates sleep homeostasis and prevents learning impairments following sleep loss. PLoS Biology, Aug. 31, 2010. doi:10.1371/journal.pbio.1000466 biology.plosjournals.org/perls… journal.pbio.1000466

Related Stories

Brain tweak lets sleep-deprived flies stay sharp

Jul 31, 2008

Staying awake slows down our brains, scientists have long recognized. Mental performance is at its peak after sleep but inevitably trends downward throughout the day, and sleep deprivation only worsens these effects.

Searching for shut eye: Study identifies possible sleep gene

Jul 29, 2008

While scientists and physicians know what happens if you don't get six to eight hours of shut-eye a night, investigators have long been puzzled about what controls the actual need for sleep. Researchers at the University ...

Biologists identify genes regulating sleeping and feeding

Jun 10, 2010

In the quest to better understand how the brain chooses between competing behaviors necessary for survival, scientists at the University of Massachusetts Medical School and New York University have isolated two genes in the ...

New research sheds light on fly sleep circuit

Nov 26, 2008

In a novel study appearing this week in Neuron, Brandeis researchers identify for the first time a specific set of wake-promoting neurons in fruit flies that are analogous to cells in the much more complex sleep circuit in hum ...

Insomniac flies resemble sleep-deprived humans

Jun 02, 2009

Researchers at Washington University School of Medicine in St. Louis have created a line of fruit flies that may someday help shed light on the mechanisms that cause insomnia in humans. The flies, which only get a small fraction ...

Recommended for you

New viral tools for mapping brains

3 hours ago

(Medical Xpress)—A brain-computer-interphase that is optogenetically-enabled is one of the most fantastic technologies we might envision today. It is likely that its full power could only be realized under ...

Link seen between seizures and migraines in the brain

19 hours ago

Seizures and migraines have always been considered separate physiological events in the brain, but now a team of engineers and neuroscientists looking at the brain from a physics viewpoint discovered a link ...

Neuroscience: Why scratching makes you itch more

Oct 30, 2014

Turns out your mom was right: Scratching an itch only makes it worse. New research from scientists at Washington University School of Medicine in St. Louis indicates that scratching causes the brain to release ...

User comments : 3

Adjust slider to filter visible comments by rank

Display comments: newest first

rgwalther
5 / 5 (1) Aug 31, 2010
Eventually starvation will put you to sleep, permanently...
Birthmark
not rated yet Aug 31, 2010
Eventually starvation will put you to sleep, permanently...


But it obviously has temporary benefits that are well worth investigating. Imagine if we could trick the mind and body into thinking it were starving? Could you imagine what this would do for school kids? :) For college kids? XD exciting advancements can be made.
Hunnter
not rated yet Sep 01, 2010
Not really. It will just cost you more money because your body won't prepare to absorb essential nutrients from the food you just ate, which you would need to rebuy over and over.

You'd probably need to eat significantly more to keep up with your bodies ignorance of the fact you have been eating.
It would be like you had a disease relating to diarrhoea where you need to eat energy dense diets and possibly, if yours is really bad, supplements.
But in this case, you'll probably end up with a lot of blockages... not fun at all.

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.