New research finds major activation themes in denture-stomatitis

Jul 16, 2010

Today during the 88th General Session & Exhibition of the International Association for Dental Research, in Barcelona, Spain, S. Offenbacher will present an abstract titled "Mucosal Gene Expression and Salivary Proteomic Analysis of Candidiasis-Associated Denture-Stomatitis."

The goal of the study was to compare whole-transcriptome, mucosal gene expression in Candida albicans (a parasitic fungus that can infect the mouth) associated chronic denture stomatitis to that of healthy oral mucosa and perform proteomic analyses of potential salivary biomarkers. Denture stomatitis is a condition in which the mucosa underneath a denture becomes inflamed and sometimes painful.

In this study, oral palatal biopsies were obtained from 17 healthy and 15 C. albicans-infected stomatitis subjects for whole-transcriptome analyses using Affymetrix arrays. The presence of C. albicans was confirmed by cytology and cultivable methods and the clinical severity of the stomatitis and denture fit evaluated by the Newton and Kapur Classifications.

A false discovery rate (FDR) of <0.05 was chosen and expression pathways associated with stomatitis were identified using ingenuity pathway analysis.

Saliva was analyzed by two-dimensional liquid chromatography and tandem mass spectrometry for proteomic identification of salivary proteins associated with oral Candidiasis.

Microarray analysis of mRNA expression revealed 3034 genes-in-play differentially expressed in C. albicans stomatitis. 235 genes were upregulated >2-fold including key cytokines [IL1F6, IL1B], chemokines [CXCL1, CCL10, IL8] as well as markers of epithelial suppression and neutrophil recruitment/ activation. Seventy-one genes were down-regulated >2-fold including epithelial adhesion molecules and keratins. Five of the 6 most significant gene ontology pathways involve inflammation and activation of the immune response with CD28 and CTLA signaling of Tcells. There was strong up-regulation of TLR2, CD14, MYD88, IKKA and NFKB as the dominant toll-like receptor signaling pathway. Six extracellular protein genes up-regulated in stomatitis were confirmed within the saliva using proteomic methods.

Neutrophil recruitment activation, epithelial suppression, TLR2 pathway up-regulation, T cell activation and bone resorption appear as major activation themes in stomatitis.

Explore further: New compounds protect nervous system from the structural damage of MS

Provided by International & American Association for Dental Research

not rated yet
add to favorites email to friend print save as pdf

Related Stories

New Way to Fight Fungal Infection

Jun 23, 2009

(PhysOrg.com) -- A team of researchers led by Amy G. Hise, M.D., M.P.H., assistant professor at the Center for Global Health and Diseases at Case Western Reserve University's School of Medicine, has discovered how the body ...

New research explores relief for xerostomia cancer patients

Jul 15, 2010

Today during the 88th General Session & Exhibition of the International Association for Dental Research, in Barcelona, Spain, presenting author S. Pradhani, University of Delaware, Newark, USA, will present an abstract titled ...

Can the tonsils influence oral HIV transmission?

Jul 26, 2007

Current research demonstrates that the tonsils may possess the necessary factors to act as a transmission site for the spread of HIV. The related report by Moutsopoulos et al, “Tonsil Epithelial Factors May Influence Oropharyngeal ...

Recommended for you

Mystery of the reverse-wired eyeball solved

Feb 27, 2015

From a practical standpoint, the wiring of the human eye - a product of our evolutionary baggage - doesn't make a lot of sense. In vertebrates, photoreceptors are located behind the neurons in the back of the eye - resulting ...

Neurons controlling appetite made from skin cells

Feb 27, 2015

Researchers have for the first time successfully converted adult human skin cells into neurons of the type that regulate appetite, providing a patient-specific model for studying the neurophysiology of weight ...

Quality control for adult stem cell treatment

Feb 27, 2015

A team of European researchers has devised a strategy to ensure that adult epidermal stem cells are safe before they are used as treatments for patients. The approach involves a clonal strategy where stem cells are collected ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.