Your fat may help you heal

Mar 25, 2010
Adipogel forms a viscous droplet when isolated on a petri dish. After further processing, it can be used as a natural extracellular matrix to support new tissue growth. PHOTO CREDIT: N. SHARMA

It frequently happens in science that what you throw away turns out to be most valuable. It happened to Deepak Nagrath, but not for long.

The Rice assistant professor in chemical and biomolecular engineering was looking for ways to grow cells in a scaffold, and he discarded the sticky substance secreted by the cells.

"I thought it was contamination, so I threw the plates away," said Nagrath, then a research associate at Harvard Medical School.

That substance, derived from adipose cells -- aka body fat -- turned out to be a natural extracellular matrix, the very thing he was looking for.

Nagrath, who joined Rice in 2009, and his co-authors have since built a biological scaffold that allows cells to grow and mature. He hopes the new material, when suffused with stem cells, will someday be injected into the human body, where it can repair tissues of many types without fear of rejection.

The research by Nagrath and his co-authors appeared last week in the Federation of American Societies for Experimental Biology (FASEB) Journal.

The basic idea is simple: Prompt fat cells to secrete what bioengineers call "basement membrane." This membrane mimics the architecture tissues naturally use in cell growth, literally a framework to which cells attach while they form a network. When the cells have matured into the desired tissue, they secrete another substance that breaks down and destroys the scaffold.

Structures that support the growth of living cells into tissues are highly valuable to pharmaceutical companies for testing drugs in vitro. Companies commonly use Matrigel, a protein mixture secreted by mouse cancer cells, but for that reason it can't be injected into patients.

"Fat is one thing that is in excess in the body. We can always lose it," Nagrath said. The substance derived from the , called Adipogel, has proven effective for growing hepatocytes, the primary liver cells often used for pharmaceutical testing.

"My approach is to force the cells to secrete a natural matrix," he said. That matrix is a honey-like gel that retains the natural growth factors, cytokines (substances that carry signals between cells) and hormones in the original tissue.

Nagrath's strategy for growing cells isn't the only approach being pursued, even at Rice: Another method reported last week in Nature Nanotechnology uses magnetic levitation to grow three-dimensional cell cultures.

But Nagrath is convinced his strategy is ultimately the most practical for rebuilding tissue in vivo, and not only because it may cost significantly less than Matrigel. "The short-term goal is to use this as a feeder layer for human embryonic stem cells. It's very hard to maintain them in the pluripotent state, where they keep dividing and are self-renewing," he said.

Once that goal is achieved, Adipogel may be just the ticket for transplanting cells to repair organs. "You can use this matrix as an adipogenic scaffold for and transplant it into the body where an organ is damaged. Then, we hope, these and the Adipogel can take over and improve their functionality."

Explore further: Two-armed control of ATR, a master regulator of the DNA damage checkpoint

More information: Read the paper at: www.fasebj.org/cgi/content/abstract/fj.09-135095v1

Related Stories

Scientists create 3-D scaffold for growing stem cells

Dec 27, 2006

Stem cells grew, multiplied and differentiated into brain cells on a new three-dimensional scaffold of tiny protein fragments designed to be more like a living body than any other cell culture system.

Scientists progress in successful tissue engineering

Mar 23, 2007

Tissue engineering is a relatively new field of basic and clinical science that is concerned, in part, with creating tissues that can augment or replace injured, defective, or diseased body parts.

Recommended for you

Japanese scientist resigns over stem cell scandal

Dec 19, 2014

A researcher embroiled in a fabrication scandal that has rocked Japan's scientific establishment said Friday she would resign after failing to reproduce results of what was once billed as a ground-breaking study on ...

'Hairclip' protein mechanism explained

Dec 18, 2014

Research led by the Teichmann group on the Wellcome Genome Campus has identified a fundamental mechanism for controlling protein function. Published in the journal Science, the discovery has wide-ranging implications for bi ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.