Scientists at Carnegie Mellon University's Department of Chemistry and Molecular Biosensor and Imaging Center (MBIC) are advancing the state-of-the-art in live cell fluorescent imaging by developing a new class of fluorescent probes that span the spectrum -- from violet to the near-infrared. The new technology, called fluoromodules, can be used to monitor biological activities of individual proteins in living cells in real time.
At the 239th national meeting of the American Chemical Society, Carnegie Mellon chemists and MBIC scientists will discuss recent advances in their fluoromodule technology that have produced diverse and photostable probes.
Fluoromodules, which consist of dye-protein complexes, provide alternatives to common fluorescent proteins, such as Green Fluorescent Protein (GFP), but with a wider selection of colors and the potential for significantly greater photostability, which allows scientists to image the dye for longer periods of time. This is made possible by the fact that the dye is noncovalently bound to the protein, which allows fresh dye to replace bleached dye.
"We initially isolated and characterized fluoromodules that generate fluorescence from the fluorogenic dyes thiazole orange and malachite green. We are now expanding our repertoire by synthesizing new dyes that emit in the orange and violet regions of the spectrum, and engineering proteins that bind to the new dyes with great affinity," said Chemistry Professor Bruce Armitage, co-director of the Center for Nucleic Acid Science and Technology at Carnegie Mellon and a member of the MBIC team developing the fluoromodules.
Fluoromodules are made of a fluorogen-activating protein (FAP) and a non-fluorescent dye called a fluorogen. The FAP, which is genetically expressed in a cell and tagged to a protein of interest, does not become fluorescent until it binds with its fluorogen. With the novel FAPs and associated fluorogens created by the MBIC team, the researchers can control when a target protein lights up, allowing them to track proteins on the cell surface and within living cells in very simple and direct ways, eliminating cumbersome experimental steps.
Recent advances in the MBIC fluoromodule technology being presented at the ACS meeting include:
- Working with a FAP that had a low affinity for the fluorogenic dye dimethlindole red (DIR), graduate student Hayriye Özhalici-Ünal used PCR mutagenesis to introduce mutations into the FAP's genetic sequence. A small number of mutations increased several-fold the protein's affinity for DIR, enabling very specific and selective binding of the FAP to its dye partner (DIR). Özhalici-Ünal will present this work at 9:50 a.m., Thursday, March 25 during the Follow-on Biologics: Protein Engineering session located in room 201 West Bldg. in the Moscone Center.
- Graduate student Nathaniel Shank synthesized a modified DIR, making it eight-times more photostable. This significant improvement could have an impact on single molecule imaging. Additionally, the modified DIR emits in the orange range of the spectrum, adding another color to the fluoromodule toolkit being developed at MBIC. Shank will present this work at 8 p.m., Tuesday, March 23 during the Total Synthesis of Complex Molecules, Material Devices & Switches, Physical Organic Chemistry poster session located in Hall D of the Moscone Center.
- By synthesizing a new dye and identifying FAPs that bind to it, research chemist Gloria Silva and graduate student Kim Zanotti developed a fluoromodule that emits fluorescence in the violet, which is a welcome addition to a very limited number of probes able to emit in the violet portion of the spectrum. Zanotti will present this work a 6 p.m., Tuesday, March 23 during the poster session located in room 3009/3011 West Bldg. in the Moscone Center.
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