MIT captures single-cell response to vaccination

Nov 03, 2008
MIT captures single-cell response to vaccination
Photo / Justin Knight/Whitehead Institute

MIT engineers have painted the most detailed portrait yet of how single cells from the immune system respond to vaccination.

The work, reported in the online edition of the Proceedings of the National Academy of Sciences the week of Nov. 3, could help researchers develop and test new vaccines for diseases including HIV, fungal infections and antibiotic-resistant bacterial infections.

"We're building a toolkit which we can use to look at how an immune response develops successfully. Then we aim to use that information for reverse engineering vaccines that would invoke that same type of response," said J. Christopher Love, assistant professor of chemical engineering and senior author of the paper.

Vaccines usually consist of an inactivated virus or bacterium that provokes B cells from the immune system to generate antibodies that attack the infectious agent.

Currently, the only way to test whether a vaccine has worked is to examine a patient's blood sample for the presence of antibodies. However, such tests do not offer a comprehensive picture of the immune system's ability to fight off infection, said Love.

"We don't know the diversity of antibodies generated, and we don't know how well they're responding to the pathogen. We don't know how poised the immune system is to respond to challenges it might face," he said.

His team's new approach generates information including the number of B cells present, whether they produce antibodies, the type of antibody they produce (for example, those that promote a long or short term response), the specificity (for a target like a protein from a virus or bacterium), and affinity (strength of binding to the target).

"This is the first time that it's possible to look at the diversity of antibody responses from primary cells, and measure a full set of their molecular characteristics, directly," said Love. "This really does give you a snapshot."

Currently, three different lab tests are needed to get all of that information, and one of the tests requires a very large number of cells. The new method works with as few as 100,000 cells (or the number in a small drop of blood).

In their PNAS study, the researchers took B cells from mice that received a series of protein injections mimicking vaccination. They positioned the cells into individual containers, arranged in a dense lattice, molded into a soft rubber. Borrowing from an artistic engraving technique used for printmaking, the researchers use that array of cells to "print" the antibodies produced by the cells onto the surface of multiple identical glass slides.

Each of those slides is exposed to different concentrations of the protein used for the model vaccine, allowing the researchers to measure how strongly each antibody binds to the target. They can then map those results back to the original immune cell, pinpointing precisely which cells produced which antibodies and how strong the cell's response was.

In addition to vaccine development, the technique could be used to build a profile of a patient's immune system and its response to treatment for allergies, cancer, or infectious diseases. "You could potentially track how the immune system is responding over time," Love said.

Source: Massachusetts Institute of Technology

Explore further: Spinach could lead to alternative energy more powerful than Popeye

add to favorites email to friend print save as pdf

Related Stories

Pushing cells towards a higher pluripotency state

Jun 24, 2014

Stem cells have the unique ability to become any type of cell in the body. Given this, the possibility that they can be cultured and engineered in the laboratory makes them an attractive option for regenerative medicine. ...

Research reveals how key controller protein is switched on

Jul 10, 2014

New research has uncovered how a complex protein pivotal in the development of cancer, viral infection and autoimmune diseases is activated. The discovery answers a key question about one of the most widely-researched proteins ...

Recommended for you

A tree may have the answers to renewable energy

13 hours ago

Through an energy conversion process that mimics that of a tree, a University of Wisconsin-Madison materials scientist is making strides in renewable energy technologies for producing hydrogen.

User comments : 0