Study reveals surprising details of the evolution of protein translation

Aug 12, 2008

A new study of transfer RNA, a molecule that delivers amino acids to the protein-building machinery of the cell, challenges long-held ideas about the evolutionary history of protein synthesis.

In the study, researchers report that the dual functions of transfer RNA (reading the genetic blueprint for a protein, and adding a specific amino acid to the protein as it is formed) appear to have originated independently of one another. The new findings are detailed in the July 30 Public Library of Science (PLoS) ONE.

University of Illinois crop sciences professor Gustavo Caetano-Anollés and postdoctoral researcher Feng-Jie Sun made the discovery by looking for clues to the evolution of protein translation in the sequence and structure of transfer RNA (tRNA).

"Structure is highly conserved, capturing information that is evolutionarily deep," Caetano-Anollés said. "It was only logical to focus on transfer RNA, a molecule that is believed to be very ancient and is truly central to the entire protein synthesis machinery." During protein synthesis, tRNA's dual function is reflected in its unique

L-shaped structure. One end of the molecule decodes messenger RNA (a molecule that carries instructions for the sequence of amino acids in a protein), while the other transfers a specific amino acid to the growing protein chain.

In previous studies, scientist assumed that the two functional domains of tRNA had evolved together. Sun and Caetano-Anollés put this assumption to the test.

They began by constructing an evolutionary family tree based on the sequence and two-dimensional structures of tRNA molecules representing every domain of life (bacteria; the microbes known as archaea; and eucarya, the domain that includes animals, plants, fungi and many other organisms) as well as viruses.

There are several dozen tRNAs (each reads a specific region of the genetic blueprint for a protein and each carries only one of the 20-plus amino acids found in proteins) so the researchers looked for clues to their evolutionary histories by comparing their physical and functional traits.

They converted the unique features of the individual tRNA cloverleaf structures into coded characters, a process that allowed a computerized search for the most parsimonious (the simplest, most probable) tRNA family trees for different organismal lineages. In this way they were able to test competing evolutionary hypotheses against the data mined from the structure of the tRNA itself.

"Our findings uniquely focus on structure, the actual aspect of the molecule that encases its function," Caetano-Anollés said.

The analysis indicated that the two functions of the tRNA had different evolutionary histories, Sun said, which suggests that they were acquired at different points in time.

The study predicted that the loading of amino acids on tRNA molecules preceded the refinement of the genetic code into codons, the regions on the messenger RNA that are read by individual tRNAs.

"For the first time, we believe we make this distinction between the evolution of the genetic code (codon discovery) and the evolution of amino acid charging," Sun said.

Source: University of Illinois at Urbana-Champaign

Explore further: Western governors tout sage grouse conservation efforts

Related Stories

In a role reversal, RNAs proofread themselves

Jan 29, 2015

Building a protein is a lot like a game of telephone: information is passed along from one messenger to another, creating the potential for errors every step of the way. There are separate, specialized enzymatic ...

Recommended for you

Western governors tout sage grouse conservation efforts

26 minutes ago

A group of Western-state governors has released a report on voluntary efforts in 11 states to conserve the habitat of sage grouse as part of an effort to avoid a federal listing of the bird under the federal ...

Scientists discover new 'transformer frog' in Ecuador

16 hours ago

It doesn't turn into Prince Charming, but a new species of frog discovered in Ecuador has earned the nickname "transformer frog" for its ability to change its skin from spiny to smooth in five minutes.

Longer DNA fragments reveal rare species diversity

17 hours ago

A challenge in metagenomics is that the more commonly used sequencing machines generate data in short lengths, while short-read assemblers may not be able to distinguish among multiple occurrences of the ...

Scientists say polar bears won't thrive on land food

17 hours ago

A group of researchers say polar bears forced off melting sea ice will not find enough food to replace their current diet of fat-laden marine mammals such as seals, a conclusion that contradicts studies indicating ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.