'Fluorescent' cells give early warning for eye disease

Feb 22, 2008

Scientists at the University of Michigan have shown that their new metabolic imaging instrument can accurately detect eye disease at a very early stage. Such a device would be vision-saving because many severe eye diseases do not exhibit early warning signals before they begin to diminish vision. The testing is noninvasive and takes less than 6 minutes to administer to a patient.

In a recent study, two researchers from the U-M Kellogg Eye Center used the instrument to measure the degree to which a subtle visual condition affected six women. Victor M. Elner, M.D., Ph.D., and Howard R. Petty, Ph.D., report their findings in the February issue of Archives of Ophthalmology. The women had been recently diagnosed with pseudotumor cerebri (PTC), a condition that mimics a brain tumor and often causes increased pressure on the optic nerve that can lead to vision loss.

Because each woman’s disease was in a very early stage, the researchers could evaluate how accurately the instrument would detect vision loss as compared to several standard tests used to evaluate vision: visual fields, visual acuity, and pupillary light response. In each case the imaging instrument provided results that were equal to and often superior to the standard tests.

The study grew out of Petty and Elner’s observation that metabolic stress at the onset of disease causes certain proteins to become fluorescent. To measure the intensity of this flavoprotein autofluorescence (FA), they designed a unique imaging system equipped with state-of-the art cameras, filters, and electronic switching, together with customized imaging software and a computer interface.

Petty, a biophysicist and expert in imaging, explains why FA data is a good predictor of disease. “Autofluorescence occurs when retinal cells begin to die, often the first event in diseases like glaucoma and diabetic retinopathy,” he says. “Cell death can be observed microscopically, but not as yet though any current imaging methods. We believe this study is a big step forward toward creating a diagnostic tool that can characterize disease long before symptoms or visible signs appear.”

The women in the study were newly diagnosed with PTC and had not yet received treatment. According to standard tests they had good visual acuity, and their visual field tests indicated either subtle abnormalities or none at all. Visual field testing, used to measure the area seen by the eye, is a standard tool for evaluating eye diseases such as glaucoma.

After the standard vision tests were administered, the researchers measured FA values for the six women and the age-matched control group. All of the patients with PTC had higher FA values in the eye that was more severely affected. In fact, FA values averaged 60% greater in the more affected eye of these women. By contrast, the control group had no significant difference in FA values between their healthy eyes.

The researchers also found that FA data more accurately described the different degree of disease in each eye for a given patient, as compared to the standard vision tests.

Elner, who is an ophthalmologist and a pathologist, says that the ability to detect subtle distinctions is important. “Early treatment for eye disease is so important, and this study suggests that FA activity is a very good indicator of eye disease,” he says. “Cardiologists have long used blood pressure testing to head off heart disease. We believe that FA testing will likewise be a helpful diagnostic tool for eye doctors looking to prevent blindness.”

Elner and Petty have patented the device through the U-M Office of Technology Transfer. They are investigating its use as a screening device in diabetes and other major eye diseases.

Source: University of Michigan

Explore further: New hope for drug discovery in African sleeping sickness

add to favorites email to friend print save as pdf

Related Stories

Shaping the way to see the world

Oct 16, 2014

The proliferation of cells, in particular the orientation in which they divide, is key in regulating the shapes of tissues. However, the cellular mechanisms that govern cell proliferation and cell division orientation are ...

Father of philosophy comes under forensic scrutiny

Oct 09, 2014

After digging up the past of Joan of Arc, Richard the Lionheart and Napoleon Bonaparte, a top forensic sleuth on Friday unveiled a medical secret of Rene Descartes, a founder of modern philosophy.

Superhuman vision

Oct 07, 2014

All through his childhood, Ramesh Raskar wished fervently for eyes in the back of his head. "I had the notion that the world did not exist if I wasn't looking at it, so I would constantly turn around to see ...

Recommended for you

New York 'fully prepared' to handle Ebola case: mayor

58 minutes ago

New York's mayor said America's largest city was fully equipped to handle Ebola as authorities sought to calm fears Friday about the virus spreading, after a doctor tested positive for the disease.

Two US nurses are declared cured of Ebola

59 minutes ago

Two American nurses were declared cured of Ebola on Friday, and one was healthy enough to leave hospital and make plans to meet President Barack Obama.

Aid group: No need to isolate staff treating Ebola

1 hour ago

Doctors Without Borders insisted Friday, after one of its doctors who worked in Guinea came down with Ebola in New York, that quarantines of health workers returning from the hot zone are not necessary when ...

User comments : 0