Researchers report breakthrough in rapid malaria detection

Dec 20, 2007

A research team led by Dr. Paul Wiseman of the Departments of Physics and Chemistry at McGill University has developed a radically new technique that uses lasers and non-linear optical effects to detect malaria infection in human blood, according to a study published in the Biophysical Journal. The researchers say the new technique holds the promise of simpler, faster and far less labour-intensive detection of the malaria parasite in blood samples.

Malaria is a vector-borne infectious disease spread by parasites of the genus Plasmodium. Most common in tropical and subtropical regions, it is a global scourge with 350 to 500 million new cases – and one to three million fatalities – reported annually. Most of the fatalities are concentrated in sub-Saharan Africa, where the resources and trained personnel currently required to accurately diagnose the disease are spread the thinnest.

Current detection techniques require trained technicians to stain slides, look for the parasite’s DNA signature under the microscope, and then manually count all the visible infected cells, a labourious process dependent on the skill and availability of trained analysts. By contrast, the proposed new technique relies on a known optical effect called third harmonic generation (THG), which causes hemozoin – a crystalline substance secreted by the parasite – to glow blue when irradiated by an infrared laser.

“People who are familiar with music know about acoustic harmonics,” said Dr. Wiseman. "You have a fundamental sound frequency and then multiples of that frequency. Non-linear optical effects are similar: if you shine an intense laser beam of a specific frequency on certain types of materials, you generate multiples of the frequency. Hemozoin has a huge, non-linear optical response for the third harmonic, which causes the blue glow."

Dr. Wiseman and his colleagues now hope to adapt well-established existing technologies like fibre-optic communications lasers and fluorescent cell sorters to quickly move the technique out of the laboratory and into the field.

"We’re imagining a self-contained unit that could be used in clinics in endemic countries," said Dr. Wiseman. "The operator could inject the cell sample directly into the device, and then it would come up with a count of the total number of existing infected cells without manual intervention."

Source: McGill University

Explore further: New flexible films for touch screen applications achieve longer lasting display

add to favorites email to friend print save as pdf

Related Stories

Watches, robots suitcases: mobile gadget highlights

12 minutes ago

Tech companies showcased countless connected gadgets at the world's biggest wireless telecom fair, the Mobile World Congress in Barcelona, which wrapped up on Thursday. Here is a selection of highlights:

Cebit 2015: DIY printing custom touch-sensitive displays

2 hours ago

Computer scientists from Saarbrücken have developed a technique that could enable virtually anyone to print out customized displays of their own in future—in all shapes and sizes and onto various materials. ...

Buckybomb shows potential power of nanoscale explosives

2 hours ago

(Phys.org)—Scientists have simulated the explosion of a modified buckminsterfullerene molecule (C60), better known as a buckyball, and shown that the reaction produces a tremendous increase in temperatur ...

Recommended for you

Understanding nickel catalysis

5 hours ago

Catalysis is a chemical phenomenon that increases the rate of a chemical reaction by spending only a tiny amount of additional substance, called a catalyst. Around 90 percent of all commercially manufactured ...

Video: What does space smell like?

19 hours ago

You can see it through a telescope, or watch a documentary about it, but you can't stick your nose out and take a whiff.

Solar cells get growth boost

23 hours ago

Researchers at the Okinawa Institute of Science and Technology Graduate University's (OIST) Energy Materials and Surface Sciences Unit have found that growing a type of film used to manufacture solar cells ...

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.