Researcher develops realistic cancer growth models

Dec 05, 2007
Researcher develops realistic cancer growth models
A scanning electron microscope image of a cell-seeded PLG scaffold. Credit: Claudia Fischbach-Teschl

Scientists can only develop new cancer drugs or search for cures by testing their theories on the real thing. Traditionally, they've done so by culturing cancer cells on petri dishes or plastic slides. But those cancer cells do not behave the way they do in the body. They only partially re-create the aggressive behavior of tumors in real patients.

That is the problem that drives Claudia Fischbach-Teschl, Cornell assistant professor of biomedical engineering, whose lab creates realistic experimental tumor models, which may then lead to better drug therapies or even a cure.

"We allow cells to grow in a 3D manner and to reorganize into tissue that better mimics their behavior and composition in the body," Fischbach-Teschl explained.

On temperature-, carbon dioxide- and humidity-controlled shelves in her lab at the Baker Institute for Animal Health, Fischbach-Teschl handles vials of pinkish, sugar-based liquids that contain what look like tiny, white floating globules. But when slid under a microscope, the beads can be seen to serve as holders, or scaffolds, of hundreds of cancer cells.

These 3D scaffolds, synthetically or naturally derived polymeric substances, act as harnesses for tumor cells as they grow, allowing the tumors to develop more closely to how they grow in the body than when maintained on flat plastic surfaces.

Fischbach-Teschl's work also involves trying to figure out why cancer cells are so able to adapt to the body's environment, why they are able to survive, and what biological secretions or proteins allow these cells to reproduce at a destructive rate.

Earlier this year in research published in Nature Methods, Fischbach-Teschl and colleagues, including David J. Mooney at Harvard University, showed that tumors grown in a 3D manner are resistant to cancer treatments, such as chemotherapy. They found the tumor models' behavior more aggressive than those grown on traditional glass slides.

Furthermore, an earlier generation of 3D tumor models, grown in what is known as a Matrigel culture, is limited in its ability to develop such characteristics. Instead, the Cornell scientists grew tumors in synthetic, porous scaffolds made of a lab-created polymer called poly(lactide-co-glycolide) (PLG). The PLG scaffolds, which look like tiny, white, flat sponges, allow the tumors to grow much more aggressively than in the Matrigel.

By then injecting the tumors into mice for in-vivo analysis, the researchers discovered that the tumors pregrown in the PLG scaffolds developed into bigger and more aggressive tumors. They also were able to analyze distinct biological functions of the cells, such as the presence of secretions that might point to the cancer cells' ability to survive.

The purpose of Fischbach-Teschl's research is to gain a fundamental understanding of cancer cell behavior that can be used to develop more effective anti-cancer drug therapy.

Fischbach-Teschl collaborates with groups in four different Cornell colleges (Weill Cornell Medical College and the Colleges of Veterinary Medicine, Human Ecology and Engineering) on various projects, including one with mechanical engineering researchers and other scientists to study bone metastasis.

The scientists hypothesize that biomechanical and structural effects of the bone mediate the migration of tumors cells to different sites, not just the primary tumor.

"Using their knowledge in the regulation of bone physiology and our approach on cancer biology, we'll gain a better understanding of how interactions between bone and tumor cells may contribute to bone metastasis," she said.

Source: By Anne Ju, Cornell University

Explore further: Early hormone therapy may be safe for women's hearts

add to favorites email to friend print save as pdf

Related Stories

First cancer immunotherapy for dogs developed

Jul 04, 2014

Nearly every second dog develops cancer from the age of ten years onward. A few therapies derived from human medicine are available for dogs. A very successful form of therapy by which antibodies inhibit ...

Engineering light-controlled proteins

Jul 03, 2014

(Phys.org) —A University of Wyoming professor has engineered proteins that can be activated by near-infrared light as a way to control biological activities in deep tissues of small mammals.

Advancing medicine, layer by layer

Jul 02, 2014

Personalized cancer treatments and better bone implants could grow from techniques demonstrated by graduate students Stephen W. Morton and Nisarg J. Shah, who are both working in chemical engineering professor ...

Recommended for you

Early hormone therapy may be safe for women's hearts

9 hours ago

(HealthDay)—Healthy women at low risk of cardiovascular disease may be able to take hormone replacement therapy soon after menopause for a short time without harming their hearts, according to a new study.

Low yield for repeat colonoscopy in some patients

9 hours ago

(HealthDay)—Repeat colonoscopies within 10 years are of little benefit to patients who had no polyps found on adequate examination; however, repeat colonoscopies do benefit patients when the baseline examination was compromised, ...

Cell's recycling center implicated in division decisions

12 hours ago

Most cells do not divide unless there is enough oxygen present to support their offspring, but certain cancer cells and other cell types circumvent this rule. Researchers at The Johns Hopkins University have now identified ...

User comments : 0