Cells take risks with their identities

Jul 12, 2007

Biologists have long thought that a simple on/off switch controls most genes in human cells. Flip the switch and a cell starts or stops producing a particular protein. But new evidence suggests that this model is too simple and that our genes are more ready for action than previously thought.

Scientists in the lab of Whitehead Member Richard Young have discovered that many human genes hover between “on” and “off” in any given cell. According to the study, which appears online in Cell on July 12, these genes begin making RNA templates for proteins—a process termed transcription—but fail to finish. The templates never materialize, and the proteins never appear.

“Surprisingly, about one-third of our genes, including all the regulators of cell identity, fall into this new class,” says Young, who is also an MIT professor of biology. “It seems awfully risky for an adult cell to leave genes primed that could change its identity.”

The human body comprises more than 200 types of cells. Each cell contains the same complete set of genes, but expresses only a unique fraction of them, churning out proteins that make it a nerve or skin or white blood cell. Scientists have known for years that a cell hides the genes it doesn’t need by coiling the dormant DNA tightly around protein spools called histones. The new study, however, suggests that DNA packaging stays loose at the beginning of many inactive genes, contrary to textbook models.

Whitehead postdoctoral researchers Matthew Guenther and Stuart Levine screened the entire human genome for a chemical signature—a landmark—that corresponds with this looser DNA packaging configuration and thus with transcription initiation. They worked with embryonic stem cells, liver cells and white blood cells.

“We expected to find the landmark on 30 to 40 percent of the genes because that’s how many are active in each cell,” Guenther says. “We were shocked when it showed up on more than 75 percent of the genes in both unspecialized embryonic stem cells and specialized adult cells.”

Further experiments confirmed that the majority of inactive genes undergo “transcriptional tryouts.” They begin making RNA, but never complete the job. Apparently, most of an inactive gene remains tightly coiled around histones, which prevent the RNA transcriptional machinery from progressing along the DNA.

“These genes are like cars revving their engines before the beginning of a race,” Guenther explains. “They’re not parked in a garage with their engines off. They’re at the starting gate, waiting for a flag that says ‘go.’”

These overzealous “cars” include all the genes responsible for directing cells along particular developmental paths—master regulators that should have no reason for gearing up in healthy specialized cells. Activating such genes might cause a cell to assume new properties. This vulnerability to metamorphosis could help to explain why some cells acquire new, unhealthy states in cancer, autoimmune diseases, diabetes and other illnesses.

It could also explain why researchers—including Whitehead Member Rudolf Jaenisch, who is also an author on the latest study—were recently able to convert mouse adult skin cells to embryonic stem cells by simply introducing four key genes. Given the right signals, inactive developmental regulators primed for transcription could roar to life.

“This is a new model for regulation of the developmental regulators,” Young maintains. “It could bring us a step closer to reprogramming cells in a controlled fashion, which has important applications for regenerative medicine.”

Source: Whitehead Institute for Biomedical Research

Explore further: Living in the genetic comfort zone

add to favorites email to friend print save as pdf

Related Stories

Virus-cutting enzyme helps bacteria remember a threat

Feb 20, 2015

Bacteria may not have brains, but they do have memories, at least when it comes to viruses that attack them. Many bacteria have a molecular immune system which allows these microbes to capture and retain ...

Evolving a bigger brain with human DNA

Feb 19, 2015

The size of the human brain expanded dramatically during the course of evolution, imparting us with unique capabilities to use abstract language and do complex math. But how did the human brain get larger ...

Jumping genes have essential biological functions

Feb 19, 2015

"Alu" sequences are small repetitive elements representing about 10% of our genome. Because of their ability to move around the genome, these "jumping genes" are considered as real motors of evolution. However, they were ...

Recommended for you

New genetic technologies offer hope for white rhino

50 minutes ago

With support from the Seaver Institute, geneticists at San Diego Zoo Institute for Conservation Research are taking the initial steps in an effort to use cryopreserved cells to bring back the northern white rhino from the ...

China bans ivory carving imports for one year

5 hours ago

Beijing has imposed a one-year ban on the import of ivory carvings, amid international criticism that rapidly-growing Chinese demand could push wild African elephants to extinction within a generation.

Living in the genetic comfort zone

14 hours ago

The information encoded in the DNA of an organism is not sufficient to determine the expression pattern of genes. This fact has been known even before the discovery of epigenetics, which refers to external ...

Cats put sight over smell in finding food

16 hours ago

Cats may prefer to use their eyes rather than follow their nose when it comes to finding the location of food, according to new research by leading animal behaviourists.

User comments : 0

Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.