Cells take risks with their identities

Jul 12, 2007

Biologists have long thought that a simple on/off switch controls most genes in human cells. Flip the switch and a cell starts or stops producing a particular protein. But new evidence suggests that this model is too simple and that our genes are more ready for action than previously thought.

Scientists in the lab of Whitehead Member Richard Young have discovered that many human genes hover between “on” and “off” in any given cell. According to the study, which appears online in Cell on July 12, these genes begin making RNA templates for proteins—a process termed transcription—but fail to finish. The templates never materialize, and the proteins never appear.

“Surprisingly, about one-third of our genes, including all the regulators of cell identity, fall into this new class,” says Young, who is also an MIT professor of biology. “It seems awfully risky for an adult cell to leave genes primed that could change its identity.”

The human body comprises more than 200 types of cells. Each cell contains the same complete set of genes, but expresses only a unique fraction of them, churning out proteins that make it a nerve or skin or white blood cell. Scientists have known for years that a cell hides the genes it doesn’t need by coiling the dormant DNA tightly around protein spools called histones. The new study, however, suggests that DNA packaging stays loose at the beginning of many inactive genes, contrary to textbook models.

Whitehead postdoctoral researchers Matthew Guenther and Stuart Levine screened the entire human genome for a chemical signature—a landmark—that corresponds with this looser DNA packaging configuration and thus with transcription initiation. They worked with embryonic stem cells, liver cells and white blood cells.

“We expected to find the landmark on 30 to 40 percent of the genes because that’s how many are active in each cell,” Guenther says. “We were shocked when it showed up on more than 75 percent of the genes in both unspecialized embryonic stem cells and specialized adult cells.”

Further experiments confirmed that the majority of inactive genes undergo “transcriptional tryouts.” They begin making RNA, but never complete the job. Apparently, most of an inactive gene remains tightly coiled around histones, which prevent the RNA transcriptional machinery from progressing along the DNA.

“These genes are like cars revving their engines before the beginning of a race,” Guenther explains. “They’re not parked in a garage with their engines off. They’re at the starting gate, waiting for a flag that says ‘go.’”

These overzealous “cars” include all the genes responsible for directing cells along particular developmental paths—master regulators that should have no reason for gearing up in healthy specialized cells. Activating such genes might cause a cell to assume new properties. This vulnerability to metamorphosis could help to explain why some cells acquire new, unhealthy states in cancer, autoimmune diseases, diabetes and other illnesses.

It could also explain why researchers—including Whitehead Member Rudolf Jaenisch, who is also an author on the latest study—were recently able to convert mouse adult skin cells to embryonic stem cells by simply introducing four key genes. Given the right signals, inactive developmental regulators primed for transcription could roar to life.

“This is a new model for regulation of the developmental regulators,” Young maintains. “It could bring us a step closer to reprogramming cells in a controlled fashion, which has important applications for regenerative medicine.”

Source: Whitehead Institute for Biomedical Research

Explore further: Declining catch rates in Caribbean green turtle fishery may be result of overfishing

add to favorites email to friend print save as pdf

Related Stories

Gene removal could have implications beyond plant science

14 hours ago

(Phys.org) —For thousands of years humans have been tinkering with plant genetics, even when they didn't realize that is what they were doing, in an effort to make stronger, healthier crops that endured climates better, ...

Recommended for you

Chimpanzees prefer firm, stable beds

4 hours ago

Chimpanzees may select a certain type of wood, Ugandan Ironwood, over other options for its firm, stable, and resilient properties to make their bed, according to a study published April 16, 2014 in the open-access ...

For cells, internal stress leads to unique shapes

5 hours ago

From far away, the top of a leaf looks like one seamless surface; however, up close, that smooth exterior is actually made up of a patchwork of cells in a variety of shapes and sizes. Interested in how these ...

Adventurous bacteria

6 hours ago

To reproduce or to conquer the world? Surprisingly, bacteria also face this problem. Theoretical biophysicists at Ludwig-Maximilians-Universitaet (LMU) in Munich have now shown how these organisms should ...

User comments : 0

More news stories

Chimpanzees prefer firm, stable beds

Chimpanzees may select a certain type of wood, Ugandan Ironwood, over other options for its firm, stable, and resilient properties to make their bed, according to a study published April 16, 2014 in the open-access ...

Revealing camouflaged bacteria

A research team at the Biozentrum of the University of Basel has discovered an protein family that plays a central role in the fight against the bacterial pathogen Salmonella within the cells. The so cal ...

Down's chromosome cause genome-wide disruption

The extra copy of Chromosome 21 that causes Down's syndrome throws a spanner into the workings of all the other chromosomes as well, said a study published Wednesday that surprised its authors.