Researchers witness natural selection at work in dramatic comeback of male butterflies

Jul 12, 2007
Hypolimnas Bolina Male
Shown is a male Hypolimnas bolina, also called the Blue Moon or Great Eggfly butterfly. A male-killing bacteria has led to skewed sex ratios in populations of H. bolina in the South Pacific, but researchers have found that male butterflies on some islands have bounced back thanks to the rise of a suppressor gene. Credit: Sylvain Charlat

An international team of researchers has documented a remarkable example of natural selection in a tropical butterfly species that fought back - genetically speaking - against a highly invasive, male-killing bacteria.

Within 10 generations that spanned less than a year, the proportion of males of the Hypolimnas bolina butterfly on the South Pacific island of Savaii jumped from a meager 1 percent of the population to about 39 percent. The researchers considered this a stunning comeback and credited it to the rise of a suppressor gene that holds in check the Wolbachia bacteria, which is passed down from the mother and selectively kills males before they have a chance to hatch.

"To my knowledge, this is the fastest evolutionary change that has ever been observed," said Sylvain Charlat, lead author of the study and a post-doctoral researcher with joint appointments at the University of California, Berkeley, and University College London. "This study shows that when a population experiences very intense selective pressures, such as an extremely skewed sex ratio, evolution can happen very fast."

The researchers' findings are described in the July 13 issue of the journal Science.

Charlat pointed out that, unlike mutations that govern such traits as wing color or antennae length, a genetic change that affects the sex ratio of a population has a very wide impact on the biology of the species.

It is not yet clear whether the suppressor gene emerged from a chance mutation from within the local population, or if it was introduced by migratory Southeast Asian butterflies in which the mutation had already been established.

"We'll likely know more in three years' time when the exact location of the suppressor gene is identified," said Charlat. "But regardless of which of the two sources of the suppressor gene is correct, natural selection is the next step. The suppressor gene allows infected females to produce males, these males will mate with many, many females, and the suppressor gene will therefore be in more and more individuals over generations."

Charlat worked with Gregory Hurst, a reader in evolutionary genetics at University College London and senior author of the paper. Descriptions of all-female broods of H. bolina date back to the 1920s, but it wasn't until 2002 that Hurst and colleagues first identified Wolbachia bacteria as the culprit behind the distorted sex ratio.

"We usually think of natural selection as acting slowly, over hundreds or thousands of years," said Hurst. "But the example in this study happened in a blink of the eye, in terms of evolutionary time, and is a remarkable thing to get to observe."

The researchers noted that bacteria that selectively kill male offspring are found among a range of arthropods, so what was seen in this study may not be unusual, despite the fact that it has never before been described in the scientific literature. Previous research has revealed some of the extraordinary ways in which insects adapt to the pressures inherent when nearly all its members are of one gender.

Notably, Charlat and Hurst reported in an earlier study that, thanks to Wolbachia, when males of H. bolina, commonly known as the Blue Moon or Great Eggfly butterfly, become a rare commodity, the number of mating sessions for both males and females jumps, possibly as an attempt to sustain the population despite the odds.

Charlat added that the relationship between Wolbachia and the Blue Moon butterfly illustrates the so-called Red Queen Principle, an evolutionary term named after a scene in Lewis Carroll's famous book, "Through the Looking-Glass," in which the characters Alice and the Red Queen run faster and faster at the top of a hill, only to find that they remain in the same place.

"In essence, organisms must evolve or change to stay in the same place, whether it's a predator-prey relationship, or a parasite-host interaction," said Charlat. "In the case of H. bolina, we're witnessing an evolutionary arms race between the parasite and the host. This strengthens the view that parasites can be major drivers in evolution."

The researchers focused on the Samoan islands of Upolu and Savaii, where in 2001, males of the Blue Moon butterfly made up only 1 percent of the population. In 2006, the researchers embarked on a new survey of the butterfly after an increase in reports of male-sightings at Upolu.

They found that males that year made up about 41 percent of the Blue Moon butterfly population in Upolu. They hatched eggs from 14 females in the lab and confirmed that the male offspring from this group were surviving with sex ratios near parity. For Savaii, the population was initially 99 percent female at the beginning of 2006. By the end of the year, researchers found that males made up 39 percent of the 54 butterflies collected.

The researchers tested for the continued presence of Wolbachia in the butterflies. By mating infected females with males from a different island that did not have the suppressor gene, they also confirmed that the bacteria were still effective at killing male embryos. The male-killing ability of the bacteria emerged again after three generations. Thus, they could rule out a change in the bacteria as an explanation for the resurgence of the males in the butterfly populations studied.

Source: University of California - Berkeley

Explore further: Declining catch rates in Caribbean green turtle fishery may be result of overfishing

add to favorites email to friend print save as pdf

Related Stories

Two new butterfly species discovered in eastern US

Feb 19, 2014

Butterflies are probably best-loved insects. As such, they are relatively well studied, especially in the United States. Eastern parts of the country are explored most thoroughly. First eastern US butterfly ...

Large testicles are linked to infidelity

Jan 29, 2014

There is a clear correlation between the size of the testicles of male primates and the proneness to infidelity of females. Learn more about sex, sperm and infidelity at the anniversary exhibition Sexus.

Recommended for you

More vets turn to prosthetics to help legless pets

57 minutes ago

A 9-month-old boxer pup named Duncan barreled down a beach in Oregon, running full tilt on soft sand into YouTube history and showing more than 4 million viewers that he can revel in a good romp despite lacking ...

Chimpanzees prefer firm, stable beds

10 hours ago

Chimpanzees may select a certain type of wood, Ugandan Ironwood, over other options for its firm, stable, and resilient properties to make their bed, according to a study published April 16, 2014 in the open-access ...

For cells, internal stress leads to unique shapes

11 hours ago

From far away, the top of a leaf looks like one seamless surface; however, up close, that smooth exterior is actually made up of a patchwork of cells in a variety of shapes and sizes. Interested in how these ...

User comments : 0

More news stories

More vets turn to prosthetics to help legless pets

A 9-month-old boxer pup named Duncan barreled down a beach in Oregon, running full tilt on soft sand into YouTube history and showing more than 4 million viewers that he can revel in a good romp despite lacking ...

Revealing camouflaged bacteria

A research team at the Biozentrum of the University of Basel has discovered an protein family that plays a central role in the fight against the bacterial pathogen Salmonella within the cells. The so cal ...

New clinical trial launched for advance lung cancer

Cancer Research UK is partnering with pharmaceutical companies AstraZeneca and Pfizer to create a pioneering clinical trial for patients with advanced lung cancer – marking a new era of research into personalised medicines ...

'Chief Yahoo' David Filo returns to board

Yahoo announced the nomination of three new board members, including company co-founder David Filo, who earned the nickname and formal job title of "Chief Yahoo."