Atomic view of cellular pump reveals how bacteria send out proteins

Atomic view of cellular pump reveals how bacteria send out proteins
The pump, a single-molecule machine, (yellow coils) carries proteins through the cell membrane (pink and dark blue). Within the pump, the researchers found a strikingly large water-filled channel (light blue), a natural environment for hydrophilic proteins. Credit: Laboratory of Membrane Biology and Biophysics/The Rockefeller University

Bacteria have plenty of things to send out into world beyond their own boundaries: coordinating signals to other members of their species, poisons for their enemies, and devious instructions to manipulate host cells they have infected. Before any of this can occur, however, they must first get the shipments past their own cell membranes, and many bacteria have evolved specialized structures and systems for launching the proteins that do these jobs.

Researchers at The Rockefeller University have determined the structure of a simple but previously unexamined pump that controls the passage of proteins through a bacterial cell membrane, an achievement that offers new insight into the mechanics that allow to manipulate their environments. The results were published in Nature on July 23.

"This pump, called PCAT for peptidase-containing ATP-binding cassette transporter, is composed of a single protein, a sort of all-in-one machine capable of recognizing its cargo, processing it, then burning chemical fuel to pump that cargo out of the cell," says study author Jue Chen, William E. Ford Professor and head of the Laboratory of Membrane Biology and Biophysics. "This new atomic-level structure explains for the first time the links between these three functions."

Of the many types of molecules cells need to move into and out of their membranes, proteins are the largest. PCATs specialize in pumping proteins out of the cell, and, because they are single-molecule machines that work alone, or with two partner proteins in some bacteria, they are the simplest such systems.

Each PCAT molecule has three domains, each in duplicate: one recognizes the cargo by a tag it carries, and cuts off that tag; another binds to and burns ATP, a molecule that contains energy stored within its atomic bonds; and the third forms a channel that spans the cells membrane. Previous work had examined the structure of the first two domains, but the structure of the third, had remained a mystery, along with the details of how the components function together.

"At this point, we have no idea how many PCATs exist, although we expect they are numerous, because each specializes in a specific type of cargo. For this study, we focused on one we called PCAT1, which transports a small protein of unknown function," says first author David Yin-wei Lin, a postdoc in the lab. "To get a sense of how PCAT1 changes shape when powered by energy from ATP, we examined the structure in two states, both with and without ATP."

The team, which also included Shuo Huang, a research technician who is now a graduate student at Georgia Institute of Technology, purified and crystalized the PCAT1 protein from the heat-loving bacterium Clostridium thermocellum. To determine the structure of the crystals, they used a technique called X-ray diffraction analysis, in which a pattern produced by X-rays bounced off the crystallized protein can be used to infer the structure of the molecule.

The first structure, determined without ATP, revealed a striking feature: a large, water-filled central channel, a natural environment for a water-loving, or hydrophilic, protein. Two side openings into this channel were guarded by the cargo-recognizing domain, acting as a sort of ticket taker. Sites on this domain would recognize and clip off the cargo's tag, before ushering the into the channel.

More information: Nature DOI: 10.1038/nature14623

Journal information: Nature

Citation: Atomic view of cellular pump reveals how bacteria send out proteins (2015, July 22) retrieved 28 March 2024 from https://phys.org/news/2015-07-atomic-view-cellular-reveals-bacteria.html
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