3-D imaging provides window into living cells, no dye required

Jan 22, 2014 by Liz Ahlberg
A new 3D imaging technique for live cells uses a conventional microscope to capture image slices throughout the depth of the cell, then computationally renders them into one three-dimensional image. The technique uses no dyes or chemicals, allowing researchers to observe cells in their natural state. Credit: Gabriel Popescu

(Phys.org) —Living cells are ready for their close-ups, thanks to a new imaging technique that needs no dyes or other chemicals, yet renders high-resolution, three-dimensional, quantitative imagery of cells and their internal structures – all with conventional microscopes and white light.

Called diffraction tomography (WDT), the imaging technique opens a window into the life of a cell without disturbing it and could allow cellular biologists unprecedented insight into cellular processes, drug effects and stem cell differentiation.

The team of University of Illinois researchers, led by electrical and computer engineering and bioengineering professor Gabriel Popescu, published their results in the journal Nature Photonics.

"One main focus of imaging cells is trying to understand how they function, or how they respond to treatments, for example, during cancer therapies," Popescu said. "If you need to add dyes or contrast agents to study them, this preparation affects the cells' function itself. It interferes with your study. With our technique, we can see processes as they happen and we don't obstruct their normal behavior."

Because it uses white light, WDT can observe cells in their natural state without exposing them to chemicals, ultraviolet radiation, or mechanical forces – the three main methods used in other microscopy techniques. White light also contains a broad spectrum of wavelengths, thus bypassing the interference issues inherent in laser light – speckles, for example.

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The 3-D images are a composite of many cross-sectional images, much like an MRI or CT image. The microscope shifts its focus through the depth of the cell, capturing images of various focus planes. Then the computer uses the theoretical model and compiles the images into a coherent three-dimensional rendering.

The greatest potential of WDT, according to the researchers, is the ability to study cells in three dimensions over time. Since the cells are not altered, they can be imaged repeatedly, allowing researchers a glimpse into the dynamics of a cell as it goes about its life – or as it is treated with a new drug.

"As a cell grows we can see the change in all three dimensions," said Taewoo Kim, a graduate student and first author of the paper. "We can see the dynamics of the cell in 3-D, which hasn't been done in a quantitative manner. For example, we could see, in the span of a minute or over a cell's lifetime, how it grows and how the things in the cell move around."

"With this imaging we can tell at what scale things within the cell are transported randomly and at what scale processes are actually organized and deterministic," Popescu said. "At first glance, the dynamics looks pretty messy, but then you look at it – we stare at movies for hours and hours – and you realize it all makes sense. Everything is organized perfectly at certain scales. That's what makes a cell alive. Randomness is just nature's way to try new things."

WDT uses a component that adds onto a conventional phase contrast microscope, a common piece of equipment in biology labs, without altering the microscope itself. The researchers used conventional microscopes with the intention of making these new optics principles easily accessible for biologists. The researchers hope that this will allow rapid large-scale adoption of WDT, and Popescu founded a startup company, Phi Optics, to help achieve that goal.

In addition to biological applications, the WDT technique has implications in the broader field of optics as the researchers pushed the boundaries of physics by applying scattering theory to imaging optics.

"The physics behind this technique is another thing we were fascinated about," Kim said. "Light propagation in general is studied with approximations, but we're using almost no approximation. In a very condensed form, we can perfectly show how the light changes as it passes through the cell."

"We started on this problem two years ago, trying to formulate mathematically the sectioning effect observed in spatial light interference light microscopy (SLIM)," said Renjie Zhou, a graduate student and co-first author of the paper. "We came up with equations which eventually described WDT. The final equation is beautiful and the theory opens opportunities for solving other optics problems in a new theoretical language."

Next, the researchers hope to pursue cross-disciplinary collaborations to explore applications of WDT in biology as well as expansions of the imaging optics demonstrated in WDT. For example, they are using WDT to watch as they differentiate in hopes of better understanding how they turn into different cell types. Since stem are so sensitive, only a chemical-free, non-invasive, white-light technique such as WDT could be used to study them without adverse effects.

Explore further: Spinning-disk microscope offers window into the center of a cell

More information: "White-light diffraction tomography of unlabeled live cells," Nature Photonics, 2014.

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EWH
not rated yet Jan 22, 2014
This is rare work that both gives generalizable new math and also produces a widely usable concrete tool. This should also work for other types of broadband tomography, but even as it is now, the microscopic technique will yield advances in knowledge of cell physiology we can't yet imagine, and quite likely there will be great practical applications of that new knowledge .
Whydening Gyre
5 / 5 (1) Jan 22, 2014
It does appear to change the paradigm of changing the observed in order to be observed...

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