Team offers new insights into the nature of important human pathogen

Nov 11, 2013 by William G. Gilroy
Team offers new insights into the nature of important human pathogen
Shahriar Mobashery

(Phys.org) —New research from a team led by Shahriar Mobashery, Navari Family Chair in Life Sciences at the University of Notre Dame, offers an insight into cell wall recycling and virulence of Pseudomonas aeruginosa, an important human pathogen. The research provides a road map for how the post-genomic analyses of biochemical processes will take place to elucidate important metabolic processes.

Pseudomonas aeruginosa is the cause of the bacterial infections that are hard to treat clinically and is the infectious agent that ultimately kills by colonizing their lungs. This organism has developed an elaborate process that links recycling of its both to antibiotic resistance and to virulence. Mobashery and his team studied the functions of three homologous enzymes, AmpD, AmpDh2 and Amp3, that are present in the organism. The genomic analysis had revealed the existence of the three, but their roles were not known.

The researchers' biochemical analyses revealed that the enzyme AmpD is involved in the recycling processes, yet AmpDh2 and AmpHh3 degrade the cell wall in a manner that implicates them in by the organism. Virulence is the basis for how an organism is a difficult pathogen.

Mobashery points out that the first genomes of bacteria were completed in the mid-1990s, yet scientists are still clueless on the functions of many of the genes from the bacterial genome. He stresses that the available genetic information needs to be followed by biochemical studies, like that done by his team on the P. aeruginosa system in elucidation of the functions of complex systems in bacteria.

Explore further: Researchers uncover keys to antibiotic resistance in MRSA

More information: The research appeared in a series of three papers published in the Journal of the American Chemical Society

J. Am. Chem. Soc. 2013, 135, 4950-4953 pubs.acs.org/doi/abs/10.1021/ja400970n
J. Am. Chem. Soc. 2013, 135, 10318-10321 pubs.acs.org/doi/abs/10.1021/ja405464b
J. Am. Chem. Soc., 2013, 135, 12605-12607 pubs.acs.org/doi/abs/10.1021/ja407445x

add to favorites email to friend print save as pdf

Related Stories

New paper sheds light on bacterial cell wall recycling

Sep 08, 2008

A new paper by a team of researchers led by Shahriar Mobashery, Navari Family Professor of Life Sciences at the University of Notre Dame, provides important new insights into the process by which bacteria recycle their cell ...

Breaking up the superbugs' party

Aug 13, 2013

The fight against antibiotic-resistant superbugs has taken a step forward thanks to a new discovery by scientists at The University of Nottingham.

Researchers uncover keys to antibiotic resistance in MRSA

Oct 04, 2013

(Phys.org) —University of Notre Dame researchers Shahriar Mobashery and Mayland Chang and their collaborators in Spain have published research results this week that show how methicillin-resistant Staphylococcus ...

Recommended for you

Chemical biologists find new halogenation enzyme

Sep 15, 2014

Molecules containing carbon-halogen bonds are produced naturally across all kingdoms of life and constitute a large family of natural products with a broad range of biological activities. The presence of halogen substituents ...

Protein secrets of Ebola virus

Sep 15, 2014

The current Ebola virus outbreak in West Africa, which has claimed more than 2000 lives, has highlighted the need for a deeper understanding of the molecular biology of the virus that could be critical in ...

User comments : 0