Progress against antibiotic-resistant bacterium with newly ID'd protein

November 27, 2013 by Jen Salazar
In a 3D structure of the protein, the binding site is shown in pink, representing a potential drug target. The green molecule shows binding of an antibiotic to the protein. Credit: Wladek Minor

( —Researchers have made inroads into tackling a bacterium that plagues hospitals and is highly resistant to most antibiotics. They determined the 3-D structure and likely function of a new protein in this common bacterium that attacks those with compromised immune systems.

The international team of scientists led by Wayne Anderson of Northwestern University and Wladek Minor of the University of Virginia also found a potential way to slow the bacteria's growth. Using the U.S. Department of Energy's Advanced Photon Source at Argonne National Laboratory, they determined that the antibiotic cephalosporin inhibits the new protein's performance and potentially could be used to develop an effective treatment.

The bacterium, called Pseudomonas aeruginosa, is widely distributed in soil, water, skin and most man-made environments throughout the world, including hospitals. It is a versatile survival expert, tolerating diverse surroundings, low-oxygen conditions and a wide variety of food sources. Because patients with weak immune systems are more susceptible to infection, this bacterium is frequently responsible for infections acquired while in hospitals—especially susceptible are burn victims and cystic fibrosis patients.

Using X-ray technology, scientists determined the detailed three-dimensional structure and molecular function of this important protein. This effort puts a face to the name of a protein previously known only by its DNA sequence. While valuable information, the DNA sequence alone is not enough to find out what a protein can do. In order to determine the function, a precise 3-D model of the protein is needed.

To characterize the new protein, known as PA4794, Karolina Majorek, Misty Kuhn and Maksymlian Chruszcz, led by Professors Anderson and Minor, analyzed enormous amounts of data. Their collaboration involved the University of Virginia, Adam Mickiewicz University in Poland, Northwestern University and the University of South Carolina, as well as the Center for Structural Genomics of Infectious Diseases and the Midwest Center of Structural Genomics, both based at Argonne. The work "Structural, functional and inhibition studies of GNAT superfamily protein PA4794: a new C-terminal lysine protein acetyltransferase form Pseudomonas aeruginosa" was published in the Journal of Biological Chemistry.

The new protein lies within some of the least explored territory in biology, inside an enormous and poorly understood superfamily of enzymes. This particular group of proteins, called the GNAT family, is found in animals, plants and bacteria. GNATs play a role in small molecule and protein acetylation, which is an important force in defining and changing the function of proteins. Proteins perform a vast array of tasks including catalyzing metabolic reactions, replicating DNA, transporting molecules from one place to another and responding to stimuli. Understanding and function is crucial for unraveling how these processes work and for discovering new drugs to control or affect these processes.

The APS facility, where the protein was described, is used by thousands of scientists worldwide. The current study used multiple beamlines run by two collaborative access teams at the APS: the Life Science-CAT and the Structural Biology Center. This large synchrotron facility has 63 beamlines available for use, each optimized for a particular field of research.

Minor said this work was "pushing beamlines to the limit of the experimental envelope." The beamlines at the APS are "the best in the world" for this type of discovery, he added, as they give the highest resolution to distinguish minute features. "Obtaining high-quality structures," he explained, "is essential to being able to determine , as the function depends on very small details."

The starting point of these studies is the preparation of suitable crystals of potential complexes of the protein, each bound to hundreds of chemical compounds called "ligands." Majorek and team analyzed several hundred datasets and screened more than 600 tiny protein crystals using a method called X-ray crystallography. Obtaining this type of data will become faster and easier in the future. When the Advanced Protein Crystallization Facility opens adjacent to the APS, we will be able to "get results on the fly, quickly redesign and re-planning experiments," Minor said. The future of discovery and the X-ray beamlines of the APS will both be brighter.

Explore further: Protein biologists find new chink in staph's armor

Related Stories

Protein biologists find new chink in staph's armor

March 25, 2011

( -- The battle against deadly staph infections is closer to victory as Illinois researchers have uncovered secrets of how the bacterium protects itself from human immune attacks, which could lead to more effective ...

Tiny crystals could revolutionize structural biology studies

November 20, 2013

For structural biologists, the first step in determining a protein's precise molecular structure is often the hardest: coaxing the protein to grow into the orderly, three-dimensional crystals that are the starting material ...

Five-dimensional crystallography probes molecular structure

November 26, 2013

( —Successful development of new pharmaceuticals could be the payoff from five-dimensional crystallography, a new experimental technique employed by researchers carrying out studies at the BioCARS facility at the ...

Recommended for you

Moonlighting molecules: Finding new uses for old enzymes

November 27, 2015

A collaboration between the University of Cambridge and MedImmune, the global biologics research and development arm of AstraZeneca, has led researchers to identify a potentially significant new application for a well-known ...

A new form of real gold, almost as light as air

November 25, 2015

Researchers at ETH Zurich have created a new type of foam made of real gold. It is the lightest form ever produced of the precious metal: a thousand times lighter than its conventional form and yet it is nearly impossible ...

Getting under the skin of a medieval mystery

November 23, 2015

A simple PVC eraser has helped an international team of scientists led by bioarchaeologists at the University of York to resolve the mystery surrounding the tissue-thin parchment used by medieval scribes to produce the first ...


Please sign in to add a comment. Registration is free, and takes less than a minute. Read more

Click here to reset your password.
Sign in to get notified via email when new comments are made.